2brs
From Proteopedia
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==Overview== | ==Overview== | ||
The eosinophil major basic protein (EMBP), a constituent of the eosinophil, secondary granule, is implicated in cytotoxicity and mediation of allergic, disorders such as asthma. It is a member of the C-type lectin family, but, lacks a Ca(2+)- and carbohydrate-binding site as seen in other members of, this family. Here, we report the crystal structure of EMBP in complex with, a heparin disaccharide and in the absence of Ca(2+), the first such report, of any C-lectin with this sugar. We also provide direct evidence of, binding of EMBP to heparin and heparin disaccharide by surface plasmon, resonance. We propose that the sugars recognized by EMBP are likely to be, proteoglycans such as heparin, leading to new interpretations for EMBP, function. | The eosinophil major basic protein (EMBP), a constituent of the eosinophil, secondary granule, is implicated in cytotoxicity and mediation of allergic, disorders such as asthma. It is a member of the C-type lectin family, but, lacks a Ca(2+)- and carbohydrate-binding site as seen in other members of, this family. Here, we report the crystal structure of EMBP in complex with, a heparin disaccharide and in the absence of Ca(2+), the first such report, of any C-lectin with this sugar. We also provide direct evidence of, binding of EMBP to heparin and heparin disaccharide by surface plasmon, resonance. We propose that the sugars recognized by EMBP are likely to be, proteoglycans such as heparin, leading to new interpretations for EMBP, function. | ||
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| + | ==Disease== | ||
| + | Known diseases associated with this structure: Chronic infections, due to MBL deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Diabetes mellitus, gestational, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Mannose-binding protein deficiency OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]], Meningococcal disease, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=154545 154545]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: signal]] | [[Category: signal]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 21:05:48 2007'' |
Revision as of 18:59, 12 November 2007
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EMBP HEPARIN COMPLEX
Contents |
Overview
The eosinophil major basic protein (EMBP), a constituent of the eosinophil, secondary granule, is implicated in cytotoxicity and mediation of allergic, disorders such as asthma. It is a member of the C-type lectin family, but, lacks a Ca(2+)- and carbohydrate-binding site as seen in other members of, this family. Here, we report the crystal structure of EMBP in complex with, a heparin disaccharide and in the absence of Ca(2+), the first such report, of any C-lectin with this sugar. We also provide direct evidence of, binding of EMBP to heparin and heparin disaccharide by surface plasmon, resonance. We propose that the sugars recognized by EMBP are likely to be, proteoglycans such as heparin, leading to new interpretations for EMBP, function.
Disease
Known diseases associated with this structure: Chronic infections, due to MBL deficiency OMIM:[154545], Diabetes mellitus, gestational, susceptibility to OMIM:[154545], Mannose-binding protein deficiency OMIM:[154545], Meningococcal disease, susceptibility to OMIM:[154545]
About this Structure
2BRS is a Single protein structure of sequence from Homo sapiens with SO4 as ligand. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Eosinophil-granule major basic protein, a C-type lectin, binds heparin., Swaminathan GJ, Myszka DG, Katsamba PS, Ohnuki LE, Gleich GJ, Acharya KR, Biochemistry. 2005 Nov 1;44(43):14152-8. PMID:16245931
Page seeded by OCA on Mon Nov 12 21:05:48 2007
Categories: Homo sapiens | Single protein | Acharya, K.R. | Gleich, G.J. | Katsamba, P.S. | Myszka, D.G. | Ohnuki, L.E. | Swaminathan, G.J. | SO4 | Antibiotic | Embp | Eosinophil | Eosinophil granule protein | Glycoprotein | Heparin | Heparin-binding | Immune response | Lectin | Proteoglycan | Signal
