1ygm
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1ygm.gif|left|200px]] | [[Image:1ygm.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1ygm", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1ygm| PDB=1ygm | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''NMR structure of Mistic''' | '''NMR structure of Mistic''' | ||
| Line 31: | Line 28: | ||
[[Category: Roosild, T P.]] | [[Category: Roosild, T P.]] | ||
[[Category: Vega, M.]] | [[Category: Vega, M.]] | ||
| - | [[Category: | + | [[Category: Alpha-helical bundle]] |
| - | [[Category: | + | [[Category: Integral membrane protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:17:35 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:17, 3 May 2008
NMR structure of Mistic
Overview
Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.
About this Structure
1YGM is a Single protein structure of sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA.
Reference
NMR structure of Mistic, a membrane-integrating protein for membrane protein expression., Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S, Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457 Page seeded by OCA on Sat May 3 16:17:35 2008
