1yiz

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[[Image:1yiz.gif|left|200px]]
[[Image:1yiz.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yiz |SIZE=350|CAPTION= <scene name='initialview01'>1yiz</scene>, resolution 1.55&Aring;
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The line below this paragraph, containing "STRUCTURE_1yiz", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= KAT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7159 Aedes aegypti])
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-->
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|DOMAIN=
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{{STRUCTURE_1yiz| PDB=1yiz | SCENE= }}
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|RELATEDENTRY=[[1yiy|1YIY]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yiz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yiz OCA], [http://www.ebi.ac.uk/pdbsum/1yiz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yiz RCSB]</span>
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}}
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'''Aedes aegypti kynurenine aminotrasferase'''
'''Aedes aegypti kynurenine aminotrasferase'''
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[[Category: Robinson, H.]]
[[Category: Robinson, H.]]
[[Category: Wilson, S.]]
[[Category: Wilson, S.]]
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[[Category: aede]]
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[[Category: Aede]]
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[[Category: kynurenic acid]]
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[[Category: Kynurenic acid]]
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[[Category: kynurenine]]
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[[Category: Kynurenine]]
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[[Category: kynurenine aminotransferase]]
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[[Category: Kynurenine aminotransferase]]
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[[Category: mosquito]]
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[[Category: Mosquito]]
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[[Category: plp]]
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[[Category: Plp]]
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[[Category: plp-enzyme]]
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[[Category: Plp-enzyme]]
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[[Category: pyridoxal phosphate]]
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[[Category: Pyridoxal phosphate]]
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[[Category: transferase]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:22:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:08:58 2008''
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Revision as of 13:22, 3 May 2008

Image:1yiz.gif

Template:STRUCTURE 1yiz

Aedes aegypti kynurenine aminotrasferase


Overview

Aedes aegypti kynurenine aminotransferase (AeKAT) catalyzes the irreversible transamination of kynurenine to kynurenic acid, the natural antagonist of NMDA and 7-nicotinic acetycholine receptors. Here, we report the crystal structure of AeKAT in its PMP and PLP forms at 1.90 and 1.55 A, respectively. The structure was solved by a combination of single-wavelength anomalous dispersion and molecular replacement approaches. The initial search model in the molecular replacement method was built with the result of single-wavelength anomalous dispersion data from the Br-AeKAT crystal in combination with homology modeling. The solved structure shows that the enzyme is a homodimer, and that the two subunits are stabilized by a number of hydrogen bonds, salts bridges, and hydrophobic interactions. Each subunit is divided into an N-terminal arm and small and large domains. Based on its folding, the enzyme belongs to the prototypical fold type, aminotransferase subgroup I. The three-dimensional structure shows a strictly conserved 'PLP-phosphate binding cup' featuring PLP-dependent enzymes. The interaction between Cys284 (A) and Cys284 (B) is unique in AeKAT, which might explain the cysteine effect of AeKAT activity. Further mutation experiments of this residue are needed to eventually understand the mechanism of the enzyme modulation by cysteine.

About this Structure

1YIZ is a Single protein structure of sequence from Aedes aegypti. Full crystallographic information is available from OCA.

Reference

Crystal structures of Aedes aegypti kynurenine aminotransferase., Han Q, Gao YG, Robinson H, Ding H, Wilson S, Li J, FEBS J. 2005 May;272(9):2198-206. PMID:15853804 Page seeded by OCA on Sat May 3 16:22:38 2008

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