1ykh
From Proteopedia
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'''Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex''' | '''Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex''' | ||
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[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Hoeppner, S.]] | [[Category: Hoeppner, S.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:26:10 2008'' | |
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Revision as of 13:26, 3 May 2008
Structure of the mediator MED7/MED21 (Med7/Srb7) subcomplex
Overview
The Mediator of transcriptional regulation is the central coactivator that enables a response of RNA polymerase II (Pol II) to activators and repressors. We present the 3.0-A crystal structure of a highly conserved part of the Mediator, the MED7.MED21 (Med7.Srb7) heterodimer. The structure is very extended, spanning one-third of the Mediator length and almost the diameter of Pol II. It shows a four-helix bundle domain and a coiled-coil protrusion connected by a flexible hinge. Four putative protein binding sites on the surface allow for assembly of the Mediator middle module and for binding of the conserved subunit MED6, which is shown to bridge to the Mediator head module. A flexible MED6 bridge and the MED7.MED21 hinge could account for changes in overall Mediator structure upon binding to Pol II or activators. Our results support the idea that transcription regulation involves conformational changes within the general machinery.
About this Structure
1YKH is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer., Baumli S, Hoeppner S, Cramer P, J Biol Chem. 2005 May 6;280(18):18171-8. Epub 2005 Feb 14. PMID:15710619 Page seeded by OCA on Sat May 3 16:26:10 2008
