1yob
From Proteopedia
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'''C69A Flavodoxin II from Azotobacter vinelandii''' | '''C69A Flavodoxin II from Azotobacter vinelandii''' | ||
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[[Category: Pouderoyen, G van.]] | [[Category: Pouderoyen, G van.]] | ||
[[Category: Rossi, G L.]] | [[Category: Rossi, G L.]] | ||
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| - | [[Category: | + | [[Category: Azotobacter vinelandii]] |
| - | [[Category: | + | [[Category: Flavodoxin ii]] |
| - | [[Category: | + | [[Category: Non-covalently bound fmn]] |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:34, 3 May 2008
C69A Flavodoxin II from Azotobacter vinelandii
Overview
Flavodoxin II from Azotobacter vinelandii is a "long-chain" flavodoxin and has one of the lowest E1 midpoint potentials found within the flavodoxin family. To better understand the relationship between structural features and redox potentials, the oxidized form of the C69A mutant of this flavodoxin was crystallized and its three-dimensional structure determined to a resolution of 2.25 A by molecular replacement. Its overall fold is similar to that of other flavodoxins, with a central five-stranded parallel beta-sheet flanked on either side by alpha-helices. An eight-residue insertion, compared with other long-chain flavodoxins, forms a short 3(10) helix preceding the start of the alpha3 helix. The flavin mononucleotide (FMN) cofactor is flanked by a leucine on its re face instead of the more conserved tryptophan, resulting in a more solvent-accessible FMN binding site and stabilization of the hydroquinone (hq) state. In particular the absence of a hydrogen bond to the N5 atom of the oxidized FMN was identified, which destabilizes the ox form, as well as an exceptionally large patch of acidic residues in the vicinity of the FMN N1 atom, which destabilizes the hq form. It is also argued that the presence of a Gly at position 58 in the sequence stabilizes the semiquinone (sq) form, as a result, raising the E2 value in particular.
About this Structure
1YOB is a Single protein structure of sequence from Azotobacter vinelandii. Full crystallographic information is available from OCA.
Reference
A crystallographic study of Cys69Ala flavodoxin II from Azotobacter vinelandii: structural determinants of redox potential., Alagaratnam S, van Pouderoyen G, Pijning T, Dijkstra BW, Cavazzini D, Rossi GL, Van Dongen WM, van Mierlo CP, van Berkel WJ, Canters GW, Protein Sci. 2005 Sep;14(9):2284-95. PMID:16131657 Page seeded by OCA on Sat May 3 16:34:37 2008
