1yod
From Proteopedia
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'''Crystal structure of a water soluble analog of phospholamban''' | '''Crystal structure of a water soluble analog of phospholamban''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOD OCA]. | |
==Reference== | ==Reference== | ||
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15826670 15826670] | X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15826670 15826670] | ||
| - | [[Category: Protein complex]] | ||
[[Category: DeGrado, W F.]] | [[Category: DeGrado, W F.]] | ||
[[Category: North, B.]] | [[Category: North, B.]] | ||
[[Category: Slovic, A M.]] | [[Category: Slovic, A M.]] | ||
[[Category: Stayrook, S E.]] | [[Category: Stayrook, S E.]] | ||
| - | [[Category: | + | [[Category: Protein design]] |
| - | [[Category: | + | [[Category: Water-soluble]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:34:43 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:34, 3 May 2008
Crystal structure of a water soluble analog of phospholamban
Overview
Phospholamban (PLB) is a pentameric transmembrane protein that regulates the Ca(2+)-dependent ATPase SERCA2a in sarcoplasmic reticulum membranes. We previously described the computational design of a water-soluble variant of phospholamban, WSPLB, which reproduced many of the structural and functional properties of the native membrane-soluble protein. While the full-length WSPLB forms a pentamer in solution, a truncated variant forms very stable tetramers. To obtain insight into the tetramer-pentamer cytoplasmic switch, we solved the crystal structure of the truncated construct, WSPLB 21-52. This peptide has a heptad sequence repeat with Leu residues at a- and Ile at d-positions from residues 31-52. The crystal structure revealed that WSPLB 21-52 adopted an antiparallel tetrameric coiled coil. This topology contrasts with the parallel topology of an analogue of the coiled-coil of GCN4 with the same Leu(a) Ile(d) repeat. Analysis of these structures revealed how the nature of the partially exposed residues at e- and g-positions influence the topology formed by the bundle. We also constructed a model for the pentameric form of PLB using the coiled-coil parameters derived from a single monomer in the tetrameric structure. This model suggests that both buried and interfacial hydrogen bonds are important for stabilizing the parallel pentamer.
About this Structure
Full crystallographic information is available from OCA.
Reference
X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils., Slovic AM, Stayrook SE, North B, Degrado WF, J Mol Biol. 2005 May 6;348(3):777-87. PMID:15826670 Page seeded by OCA on Sat May 3 16:34:43 2008
