1yoe

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[[Image:1yoe.gif|left|200px]]
[[Image:1yoe.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yoe |SIZE=350|CAPTION= <scene name='initialview01'>1yoe</scene>, resolution 1.78&Aring;
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The line below this paragraph, containing "STRUCTURE_1yoe", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=RIB:RIBOSE'>RIB</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribosylpyrimidine_nucleosidase Ribosylpyrimidine nucleosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.8 3.2.2.8] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ybeK/rihA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1yoe| PDB=1yoe | SCENE= }}
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|RELATEDENTRY=[[1mas|1MAS]], [[2mas|2MAS]], [[1ezr|1EZR]], [[1q8f|1Q8F]], [[1hoz|1HOZ]], [[1kie|1KIE]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yoe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yoe OCA], [http://www.ebi.ac.uk/pdbsum/1yoe PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yoe RCSB]</span>
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}}
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'''Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose'''
'''Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose'''
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[[Category: Steyaert, J.]]
[[Category: Steyaert, J.]]
[[Category: Versees, W.]]
[[Category: Versees, W.]]
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[[Category: bacterial nucleosidase]]
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[[Category: Bacterial nucleosidase]]
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[[Category: enzyme/product complex]]
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[[Category: Enzyme/product complex]]
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[[Category: pyrimidine nucleoside hydrolase]]
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[[Category: Pyrimidine nucleoside hydrolase]]
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[[Category: ribose]]
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[[Category: Ribose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:34:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:15:36 2008''
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Revision as of 13:34, 3 May 2008

Template:STRUCTURE 1yoe

Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose


Overview

Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment.

About this Structure

1YOE is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy., Giabbai B, Degano M, Structure. 2004 May;12(5):739-49. PMID:15130467 Page seeded by OCA on Sat May 3 16:34:48 2008

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