1ypv

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[[Image:1ypv.gif|left|200px]]
[[Image:1ypv.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1ypv |SIZE=350|CAPTION= <scene name='initialview01'>1ypv</scene>, resolution 1.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1ypv", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SCH:S-METHYL+THIOCYSTEINE+GROUP'>SCH</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thymidylate_synthase Thymidylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.45 2.1.1.45] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TYMS, TS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1ypv| PDB=1ypv | SCENE= }}
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|RELATEDENTRY=[[1hw3|1HW3]], [[1hvy|1HVY]], [[1hw4|1HW4]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ypv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ypv OCA], [http://www.ebi.ac.uk/pdbsum/1ypv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ypv RCSB]</span>
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}}
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'''Structure of human thymidylate synthase at low salt conditions'''
'''Structure of human thymidylate synthase at low salt conditions'''
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[[Category: Lovelace, L L.]]
[[Category: Lovelace, L L.]]
[[Category: Minor, W.]]
[[Category: Minor, W.]]
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[[Category: methyltransferase]]
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[[Category: Methyltransferase]]
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[[Category: thymidylate synthase]]
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[[Category: Thymidylate synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:37:45 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:17:14 2008''
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Revision as of 13:37, 3 May 2008

Image:1ypv.gif

Template:STRUCTURE 1ypv

Structure of human thymidylate synthase at low salt conditions


Overview

Human thymidylate synthase, a target in cancer chemotherapy, was crystallized from PEG 3350 with 30 mM ammonium sulfate (AS) in the crystallization medium. The crystals are isomorphous with the high-salt crystals ( approximately 2.0 M AS) and the structure has been solved and refined (R = 22.6%, R(free) = 24.3%) at 1.8 A resolution. The high- and low-AS-concentration structures are quite similar, with loop 181-197 is in the inactive conformation. Also, residues 95-106 and 129-135 (eukaryotic inserts region) show high mobility as assessed by poor electron density and high values of crystallographic temperature factors (residues 1-25 and 108-129 are disordered in both structures). The high mobility of this region may reflect the situation at physiological ionic strength. Of the four sulfate ions observed bound at 2.0 M AS, only two are present at 30 mM AS. The inactive conformation appears to be stabilized by the side chain of Val3 or a leucine residue from the disordered regions. The low-salt conditions of these crystals should be much more suitable for the study of thymidylate synthase inhibitors, especially those that utilize sulfate-binding sites to stabilize the inactive conformation of loop 181-197.

About this Structure

1YPV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of human thymidylate synthase under low-salt conditions., Lovelace LL, Minor W, Lebioda L, Acta Crystallogr D Biol Crystallogr. 2005 May;61(Pt 5):622-7. Epub 2005, Apr 20. PMID:15858273 Page seeded by OCA on Sat May 3 16:37:45 2008

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