1yq2

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[[Image:1yq2.gif|left|200px]]
[[Image:1yq2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yq2 |SIZE=350|CAPTION= <scene name='initialview01'>1yq2</scene>, resolution 1.90&Aring;
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The line below this paragraph, containing "STRUCTURE_1yq2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= LacZ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria])
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-->
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|DOMAIN=
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{{STRUCTURE_1yq2| PDB=1yq2 | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yq2 OCA], [http://www.ebi.ac.uk/pdbsum/1yq2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yq2 RCSB]</span>
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}}
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'''beta-galactosidase from Arthrobacter sp. C2-2 (isoenzyme C2-2-1)'''
'''beta-galactosidase from Arthrobacter sp. C2-2 (isoenzyme C2-2-1)'''
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[[Category: Strnad, H.]]
[[Category: Strnad, H.]]
[[Category: Vondrackova, E.]]
[[Category: Vondrackova, E.]]
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[[Category: glycosyl hydrolase family 2]]
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[[Category: Glycosyl hydrolase family 2]]
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[[Category: hexamer]]
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[[Category: Hexamer]]
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[[Category: tim barrel]]
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[[Category: Tim barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:38:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:17:24 2008''
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Revision as of 13:38, 3 May 2008

Image:1yq2.gif

Template:STRUCTURE 1yq2

beta-galactosidase from Arthrobacter sp. C2-2 (isoenzyme C2-2-1)


Overview

The X-ray structure of cold-active beta-galactosidase (isoenzyme C-2-2-1) from an Antarctic bacterium Arthrobacter sp. C2-2 was solved at 1.9A resolution. The enzyme forms 660 kDa hexamers with active sites opened to the central cavity of the hexamer and connected by eight channels with exterior solvent. To our best knowledge, this is the first cold-active beta-galactosidase with known structure and also the first known beta-galactosidase structure in the form of compact hexamers. The hexamer organization regulates access of substrates and ligands to six active sites and this unique packing, present also in solution, raises questions about its purpose and function. This enzyme belongs to glycosyl hydrolase family 2, similarly to Escherichia coli beta-galactosidase, forming tetramers necessary for its enzymatic function. However, we discovered significant differences between these two enzymes affecting the ability of tetramer/hexamer formation and complementation of the active site. This structure reveals new insights into the cold-adaptation mechanisms of enzymatic pathways of extremophiles.

About this Structure

1YQ2 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Cold-active beta-galactosidase from Arthrobacter sp. C2-2 forms compact 660 kDa hexamers: crystal structure at 1.9A resolution., Skalova T, Dohnalek J, Spiwok V, Lipovova P, Vondrackova E, Petrokova H, Duskova J, Strnad H, Kralova B, Hasek J, J Mol Biol. 2005 Oct 21;353(2):282-94. PMID:16171818 Page seeded by OCA on Sat May 3 16:38:04 2008

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