1yql

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[[Image:1yql.gif|left|200px]]
[[Image:1yql.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yql |SIZE=350|CAPTION= <scene name='initialview01'>1yql</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1yql", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PPW:7-DEAZA-8-AZA-2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>PPW</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= OGG1, MMH, MUTM, OGH1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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-->
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|DOMAIN=
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{{STRUCTURE_1yql| PDB=1yql | SCENE= }}
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|RELATEDENTRY=[[1yqk|1YQK]], [[1ebm|1EBM]], [[1yqr|1YQR]], [[1yqm|1YQM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yql FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yql OCA], [http://www.ebi.ac.uk/pdbsum/1yql PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yql RCSB]</span>
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}}
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'''Catalytically inactive hOGG1 crosslinked with 7-deaza-8-azaguanine containing DNA'''
'''Catalytically inactive hOGG1 crosslinked with 7-deaza-8-azaguanine containing DNA'''
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[[Category: Verdine, G L.]]
[[Category: Verdine, G L.]]
[[Category: Yang, W.]]
[[Category: Yang, W.]]
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[[Category: disulfide crosslinking]]
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[[Category: Disulfide crosslinking]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:39:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:18:05 2008''
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Revision as of 13:39, 3 May 2008

Image:1yql.gif

Template:STRUCTURE 1yql

Catalytically inactive hOGG1 crosslinked with 7-deaza-8-azaguanine containing DNA


Overview

How DNA repair proteins distinguish between the rare sites of damage and the vast expanse of normal DNA is poorly understood. Recognizing the mutagenic lesion 8-oxoguanine (oxoG) represents an especially formidable challenge, because this oxidized nucleobase differs by only two atoms from its normal counterpart, guanine (G). Here we report the use of a covalent trapping strategy to capture a human oxoG repair protein, 8-oxoguanine DNA glycosylase I (hOGG1), in the act of interrogating normal DNA. The X-ray structure of the trapped complex features a target G nucleobase extruded from the DNA helix but denied insertion into the lesion recognition pocket of the enzyme. Free energy difference calculations show that both attractive and repulsive interactions have an important role in the preferential binding of oxoG compared with G to the active site. The structure reveals a remarkably effective gate-keeping strategy for lesion discrimination and suggests a mechanism for oxoG insertion into the hOGG1 active site.

About this Structure

1YQL is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a repair enzyme interrogating undamaged DNA elucidates recognition of damaged DNA., Banerjee A, Yang W, Karplus M, Verdine GL, Nature. 2005 Mar 31;434(7033):612-8. PMID:15800616 Page seeded by OCA on Sat May 3 16:39:22 2008

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OCA

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