1yry

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[[Image:1yry.gif|left|200px]]
[[Image:1yry.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1yry |SIZE=350|CAPTION= <scene name='initialview01'>1yry</scene>, resolution 2.80&Aring;
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The line below this paragraph, containing "STRUCTURE_1yry", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MSG:7-METHYL-6-THIO-GUANOSINE'>MSG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Purine-nucleoside_phosphorylase Purine-nucleoside phosphorylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.1 2.4.2.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_1yry| PDB=1yry | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yry FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yry OCA], [http://www.ebi.ac.uk/pdbsum/1yry PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yry RCSB]</span>
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}}
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'''Crystal structure of human PNP complexed with MESG'''
'''Crystal structure of human PNP complexed with MESG'''
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[[Category: Santos, D S.]]
[[Category: Santos, D S.]]
[[Category: Silva, R G.]]
[[Category: Silva, R G.]]
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[[Category: crystallography]]
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[[Category: Crystallography]]
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[[Category: drug design]]
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[[Category: Drug design]]
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[[Category: mesg]]
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[[Category: Mesg]]
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[[Category: purine nucleoside phosphorylase]]
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[[Category: Purine nucleoside phosphorylase]]
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[[Category: synchrotron]]
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[[Category: Synchrotron]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:42:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:19:59 2008''
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Revision as of 13:42, 3 May 2008

Image:1yry.gif

Template:STRUCTURE 1yry

Crystal structure of human PNP complexed with MESG


Overview

Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. The lack of such an activity in humans, owing to a genetic disorder, causes T-cell impairment, and drugs that inhibit this enzyme may have the potential of being utilized as modulators of the immunological system to treat leukemia, autoimmune diseases, and rejection in organ transplantation. Here, we describe kinetics and crystal structure of human PNP in complex with 7-methyl-6-thio-guanosine, a synthetic substrate, which is largely used in activity assays. Analysis of the structure identifies different protein conformational changes upon ligand binding, and comparison of kinetic and structural data permits an understanding of the effects of atomic substitution on key positions of the synthetic substrate and their consequences to enzyme binding and catalysis. Such knowledge may be helpful in designing new PNP inhibitors.

About this Structure

1YRY is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine., Silva RG, Pereira JH, Canduri F, de Azevedo WF Jr, Basso LA, Santos DS, Arch Biochem Biophys. 2005 Oct 1;442(1):49-58. PMID:16154528 Page seeded by OCA on Sat May 3 16:42:27 2008

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