1yt6
From Proteopedia
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[[Image:1yt6.gif|left|200px]] | [[Image:1yt6.gif|left|200px]] | ||
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'''NMR structure of peptide SD''' | '''NMR structure of peptide SD''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YT6 OCA]. | |
==Reference== | ==Reference== | ||
Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand., Murata T, Hemmi H, Nakamura S, Shimizu K, Suzuki Y, Yamaguchi I, FEBS J. 2005 Oct;272(19):4938-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16176267 16176267] | Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand., Murata T, Hemmi H, Nakamura S, Shimizu K, Suzuki Y, Yamaguchi I, FEBS J. 2005 Oct;272(19):4938-48. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16176267 16176267] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hemmi, H.]] | [[Category: Hemmi, H.]] | ||
[[Category: Murata, T.]] | [[Category: Murata, T.]] | ||
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[[Category: Suzuki, Y.]] | [[Category: Suzuki, Y.]] | ||
[[Category: Yamaguchi, I.]] | [[Category: Yamaguchi, I.]] | ||
| - | [[Category: | + | [[Category: Gibberellin]] |
| - | [[Category: | + | [[Category: Mimic]] |
| - | [[Category: | + | [[Category: Std-nmr]] |
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Revision as of 13:45, 3 May 2008
NMR structure of peptide SD
Overview
Using NMR spectroscopy and simulated annealing calculations, we determined the solution structure of the disulfide-linked cyclized decapeptide ACLPWSDGPC (SD), which is bound to an anti-(gibberellin A(4)) mAb 4-B8(8)/E9 and was found to be the first peptidyl mimotope for a hydrophobic ligand. The resulting structure of the peptide showed a beta-turn-like conformation in residues three to seven and the region converges well (average rmsd 0.54 A). The binding activity and the epitopes of the peptide to the antibody were assessed using saturation transfer difference (STD)-NMR experiments. We also conducted docking simulations between the peptide and the mAb to determine how the peptide is bound to the mAb. Resonances around the beta-turn-like conformation of peptide SD (residues 3-5) showed strong STD enhancement, which agreed well with results from docking simulation between peptide SD and the mAb. Together with the commonality of amino acid residues of the mAb involved in interactions with gibberellin A(4) (GA(4)) and peptide SD, we concluded that peptide SD is bound to the antigen-binding site of mAb 4-B8(8)/E9 as a GA(4) mimic, confirming evidence for the existence of peptide mimics even for hydrophobic ligands.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure, epitope mapping, and docking simulation of a gibberellin mimic peptide as a peptidyl mimotope for a hydrophobic ligand., Murata T, Hemmi H, Nakamura S, Shimizu K, Suzuki Y, Yamaguchi I, FEBS J. 2005 Oct;272(19):4938-48. PMID:16176267 Page seeded by OCA on Sat May 3 16:45:25 2008
Categories: Hemmi, H. | Murata, T. | Nakamura, S. | Shimizu, K. | Suzuki, Y. | Yamaguchi, I. | Gibberellin | Mimic | Std-nmr
