1yuh
From Proteopedia
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[[Image:1yuh.jpg|left|200px]] | [[Image:1yuh.jpg|left|200px]] | ||
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'''FAB FRAGMENT''' | '''FAB FRAGMENT''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1YUH is a [[Protein complex]] structure | + | 1YUH is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YUH OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Strand, M.]] | [[Category: Strand, M.]] | ||
[[Category: Yuhasz, S C.]] | [[Category: Yuhasz, S C.]] | ||
| - | [[Category: | + | [[Category: Anti-nitrophenol]] |
| - | [[Category: | + | [[Category: Immunoglobulin]] |
| - | [[Category: | + | [[Category: Lambda light chain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 16:48:23 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 13:48, 3 May 2008
FAB FRAGMENT
Overview
Affinity maturation of the immune response to nitrophenol-containing antigens has been extensively investigated. Significant strides made during the past several years with the advent of PCR technology have provided a wealth of biochemical knowledge. Structural investigations of the phenomena have however been limited. We have determined the three-dimensional structure of the Fab fragment of 88C6/12, an anti-4-hydroxy-3-nitrophenyl acetic acid antibody complexed with the immunizing hapten and with a heteroclitic iodinated hapten. The crystallographic structure of the complexes reveals that the binding is stabilized by a number of hydrogen bonds and extensive van der Waals interactions between the hapten and the antibody. In addition, the Fab binding pocket contains a region of positive electrostatic potential well suited for interaction with the predominant resonance form of the nitrophenyl ring system. The observed heteroclicity towards the iodinated hapten is not a direct result of iodine-protein interactions, but results from the enhanced stability in the iodinated ring of the resonance form that binds the antibody. In addition this investigation provides a rationale for the strong preference for the substitution in the heavy chain from the germ-line gene encoded Trp 33 to Leu 33 in the mature anti-nitrophenol response.
About this Structure
1YUH is a Protein complex structure. Full crystallographic information is available from OCA.
Reference
Structural analysis of affinity maturation: the three-dimensional structures of complexes of an anti-nitrophenol antibody., Yuhasz SC, Parry C, Strand M, Amzel LM, Mol Immunol. 1995 Oct;32(14-15):1143-55. PMID:8544863 Page seeded by OCA on Sat May 3 16:48:23 2008
