1z23
From Proteopedia
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[[Image:1z23.gif|left|200px]] | [[Image:1z23.gif|left|200px]] | ||
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'''The serine-rich domain from Crk-associated substrate (p130Cas)''' | '''The serine-rich domain from Crk-associated substrate (p130Cas)''' | ||
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[[Category: Olson, A J.]] | [[Category: Olson, A J.]] | ||
[[Category: Vuori, K.]] | [[Category: Vuori, K.]] | ||
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Revision as of 14:05, 3 May 2008
The serine-rich domain from Crk-associated substrate (p130Cas)
Overview
p130(cas) (Crk-associated substrate) is a docking protein that is involved in assembly of focal adhesions and concomitant cellular signaling. It plays a role in physiological regulation of cell adhesion, migration, survival, and proliferation, as well as in oncogenic transformation. The molecule consists of multiple protein-protein interaction motifs, including a serine-rich region that is positioned between Crk and Src-binding sites. This study reports the first structure of a functional domain of Cas. The solution structure of the serine-rich region has been determined by NMR spectroscopy, demonstrating that this is a stable domain that folds as a four-helix bundle, a protein-interaction motif. The serine-rich region bears strong structural similarity to four-helix bundles found in other adhesion components like focal adhesion kinase, alpha-catenin, or vinculin. Potential sites for phosphorylation and interaction with the 14-3-3 family of cellular regulators are identified in the domain and characterized by site-directed mutagenesis and binding assays. Mapping the degree of amino acid conservation onto the molecular surface reveals a patch of invariant residues near the C terminus of the bundle, which may represent a previously unidentified site for protein interaction.
About this Structure
1Z23 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
The serine-rich domain from Crk-associated substrate (p130cas) is a four-helix bundle., Briknarova K, Nasertorabi F, Havert ML, Eggleston E, Hoyt DW, Li C, Olson AJ, Vuori K, Ely KR, J Biol Chem. 2005 Jun 10;280(23):21908-14. Epub 2005 Mar 28. PMID:15795225 Page seeded by OCA on Sat May 3 17:05:35 2008
