1z2j
From Proteopedia
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'''Solution structure of the HIV-1 frameshift inducing element''' | '''Solution structure of the HIV-1 frameshift inducing element''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2J OCA]. | |
==Reference== | ==Reference== | ||
Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15927637 15927637] | Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15927637 15927637] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Butcher, S E.]] | [[Category: Butcher, S E.]] | ||
[[Category: Staple, D W.]] | [[Category: Staple, D W.]] | ||
| - | [[Category: | + | [[Category: Purine bulge]] |
| - | [[Category: | + | [[Category: Stem-loop]] |
| - | [[Category: | + | [[Category: Tetraloop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:06:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:06, 3 May 2008
Solution structure of the HIV-1 frameshift inducing element
Overview
Expression of the HIV reverse transcriptase and other essential viral enzymes requires a -1 translational frameshift. The frameshift event is induced by two highly conserved RNA elements within the HIV-1 mRNA: a UUUUUUA heptamer known as the slippery sequence, and a downstream RNA structure. Here, we report structural and thermodynamic evidence that the HIV-1 frameshift site RNA forms a stem-loop and lower helix separated by a three-purine bulge. We have determined the structure of the 45 nucleotide frameshift site RNA using multidimensional heteronuclear nuclear magnetic resonance (NMR) methods. The upper helix is highly thermostable (T(m)>90 degrees C), forming 11 Watson-Crick base-pairs capped by a stable ACAA tetraloop. The eight base-pair lower helix was found to be only moderately stable (T(m)=47 degrees C). A three-purine bulge separates the highly stable upper helix from the lower helix. Base stacking in the bulge forms a wedge, introducing a 60 degrees bend between the helices. Interestingly, this bend is similar to those seen in a number of frameshift inducing pseudoknots for which structures have been solved. The lower helix must denature to allow the ribosome access to the slippery site, but likely functions as a positioning element that enhances frameshift efficiency.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure and thermodynamic investigation of the HIV-1 frameshift inducing element., Staple DW, Butcher SE, J Mol Biol. 2005 Jun 24;349(5):1011-23. Epub 2005 Apr 1. PMID:15927637 Page seeded by OCA on Sat May 3 17:06:32 2008
