1z43
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1z43.gif|left|200px]] | [[Image:1z43.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1z43", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1z43| PDB=1z43 | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Crystal structure of 7S.S SRP RNA of M. jannaschii''' | '''Crystal structure of 7S.S SRP RNA of M. jannaschii''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z43 OCA]. | |
==Reference== | ==Reference== | ||
Structural insights into SRP RNA: an induced fit mechanism for SRP assembly., Hainzl T, Huang S, Sauer-Eriksson AE, RNA. 2005 Jul;11(7):1043-50. Epub 2005 May 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15928341 15928341] | Structural insights into SRP RNA: an induced fit mechanism for SRP assembly., Hainzl T, Huang S, Sauer-Eriksson AE, RNA. 2005 Jul;11(7):1043-50. Epub 2005 May 31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15928341 15928341] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Hainzl, T.]] | [[Category: Hainzl, T.]] | ||
[[Category: Huang, S.]] | [[Category: Huang, S.]] | ||
[[Category: Sauer-Eriksson, A E.]] | [[Category: Sauer-Eriksson, A E.]] | ||
| - | [[Category: | + | [[Category: Rna]] |
| - | [[Category: | + | [[Category: Tetra-loop]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:09:42 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:09, 3 May 2008
Crystal structure of 7S.S SRP RNA of M. jannaschii
Overview
Proper assembly of large protein-RNA complexes requires sequential binding of the proteins to the RNA. The signal recognition particle (SRP) is a multiprotein-RNA complex responsible for the cotranslational targeting of proteins to biological membranes. Here we describe the crystal structure at 2.6-A resolution of the S-domain of SRP RNA from the archeon Methanococcus jannaschii. Comparison of this structure with the SRP19-bound form reveals the nature of the SRP19-induced conformational changes, which promote subsequent SRP54 attachment. These structural changes are initiated at the SRP19 binding site and transmitted through helix 6 to looped-out adenosines, which form tertiary RNA interaction with helix 8. Displacement of these adenosines enforces a conformational change of the asymmetric loop structure in helix 8. In free RNA, the three unpaired bases A195, C196, and C197 are directed toward the helical axis, whereas upon SRP19 binding the loop backbone inverts and the bases are splayed out in a conformation that resembles the SRP54-bound form. Nucleotides adjacent to the bulged nucleotides seem to be particularly important in the regulation of this loop transition. Binding of SRP19 to 7S RNA reveals an elegant mechanism of how protein-induced changes are directed through an RNA molecule and may relate to those regulating the assembly of other RNPs.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structural insights into SRP RNA: an induced fit mechanism for SRP assembly., Hainzl T, Huang S, Sauer-Eriksson AE, RNA. 2005 Jul;11(7):1043-50. Epub 2005 May 31. PMID:15928341 Page seeded by OCA on Sat May 3 17:09:42 2008
