1z4h
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1z4h.gif|left|200px]] | [[Image:1z4h.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1z4h", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1z4h| PDB=1z4h | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''The response regulator TorI belongs to a new family of atypical excisionase''' | '''The response regulator TorI belongs to a new family of atypical excisionase''' | ||
| Line 30: | Line 27: | ||
[[Category: Mejean, V.]] | [[Category: Mejean, V.]] | ||
[[Category: Morelli, X.]] | [[Category: Morelli, X.]] | ||
| - | [[Category: | + | [[Category: Reverse turn]] |
| - | [[Category: | + | [[Category: Winged helix]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:10:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:10, 3 May 2008
The response regulator TorI belongs to a new family of atypical excisionase
Overview
TorI (Tor inhibition protein) has been identified in Escherichia coli as a protein inhibitor acting through protein-protein interaction with the TorR response regulator. This interaction, which does not interfere with TorR DNA binding activity, probably prevents the recruitment of RNA polymerase to the torC promoter. In this study we have solved the solution structure of TorI, which adopts a prokaryotic winged-helix arrangement. Despite no primary sequence similarity, the three-dimensional structure of TorI is highly homologous to the (lambda)Xis, Mu bacteriophage repressor (MuR-DBD), and transposase (MuA-DBD) structures. We propose that the TorI protein is the structural missing link between the (lambda)Xis and MuR proteins. Moreover, in vivo assays demonstrated that TorI plays an essential role in prophage excision. Heteronuclear NMR experiments and site-directed mutagenesis studies have pinpointed out key residues involved in the DNA binding activity of TorI. Our findings suggest that TorI-related proteins identified in various pathogenic bacterial genomes define a new family of atypical excisionases.
About this Structure
1Z4H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural and genetic analyses reveal a key role in prophage excision for the TorI response regulator inhibitor., Elantak L, Ansaldi M, Guerlesquin F, Mejean V, Morelli X, J Biol Chem. 2005 Nov 4;280(44):36802-8. Epub 2005 Aug 2. PMID:16079126 Page seeded by OCA on Sat May 3 17:10:02 2008
