1z59
From Proteopedia
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[[Image:1z59.gif|left|200px]] | [[Image:1z59.gif|left|200px]] | ||
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| - | | | + | {{STRUCTURE_1z59| PDB=1z59 | SCENE= }} |
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'''Topoisomerase VI-B, ADP-bound monomer form''' | '''Topoisomerase VI-B, ADP-bound monomer form''' | ||
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==Reference== | ==Reference== | ||
Structural dissection of ATP turnover in the prototypical GHL ATPase TopoVI., Corbett KD, Berger JM, Structure. 2005 Jun;13(6):873-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15939019 15939019] | Structural dissection of ATP turnover in the prototypical GHL ATPase TopoVI., Corbett KD, Berger JM, Structure. 2005 Jun;13(6):873-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15939019 15939019] | ||
| - | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sulfolobus shibatae]] | [[Category: Sulfolobus shibatae]] | ||
[[Category: Berger, J M.]] | [[Category: Berger, J M.]] | ||
[[Category: Corbett, K D.]] | [[Category: Corbett, K D.]] | ||
| - | [[Category: | + | [[Category: Archaea]] |
| - | [[Category: | + | [[Category: Atpase]] |
| - | [[Category: | + | [[Category: Topoisomerase]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:11:32 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:11, 3 May 2008
Topoisomerase VI-B, ADP-bound monomer form
Overview
GHL proteins are functionally diverse enzymes defined by the presence of a conserved ATPase domain that self-associates to trap substrate upon nucleotide binding. The structural states adopted by these enzymes during nucleotide hydrolysis and product release, and their consequences for enzyme catalysis, have remained unclear. Here, we have determined a complete structural map of the ATP turnover cycle for topoVI-B, the ATPase subunit of the archaeal GHL enzyme topoisomerase VI. With this ensemble of structures, we show that significant conformational changes in the subunit occur first upon ATP binding, and subsequently upon release of hydrolyzed P(i). Together, these data provide a structural framework for understanding the role of ATP hydrolysis in the type II topoisomerase reaction. Our results also suggest that the GHL ATPase module is a molecular switch in which ATP hydrolysis serves as a prerequisite but not a driving force for substrate-dependent structural transitions in the enzyme.
About this Structure
1Z59 is a Single protein structure of sequence from Sulfolobus shibatae. Full crystallographic information is available from OCA.
Reference
Structural dissection of ATP turnover in the prototypical GHL ATPase TopoVI., Corbett KD, Berger JM, Structure. 2005 Jun;13(6):873-82. PMID:15939019 Page seeded by OCA on Sat May 3 17:11:32 2008
