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1z7h
From Proteopedia
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[[Image:1z7h.gif|left|200px]] | [[Image:1z7h.gif|left|200px]] | ||
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'''2.3 Angstrom crystal structure of tetanus neurotoxin light chain''' | '''2.3 Angstrom crystal structure of tetanus neurotoxin light chain''' | ||
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[[Category: Breidenbach, M A.]] | [[Category: Breidenbach, M A.]] | ||
[[Category: Brunger, A T.]] | [[Category: Brunger, A T.]] | ||
| - | [[Category: | + | [[Category: Metalloprotease]] |
| - | [[Category: | + | [[Category: Neurotransmission]] |
| - | [[Category: | + | [[Category: Snare]] |
| - | [[Category: | + | [[Category: Tent]] |
| - | [[Category: | + | [[Category: Tetanus]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:16:30 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:16, 3 May 2008
2.3 Angstrom crystal structure of tetanus neurotoxin light chain
Overview
TeNT is the causative agent of the neuroparalytic disease tetanus. A key component of TeNT is its light chain, a Zn(2+) endopeptidase that targets SNAREs. Recent structural studies of closely related BoNT endopeptidases indicate that substrate-binding exosites remote from a conserved active site are the primary determinants of substrate specificity. Here we report the 2.3 A X-ray crystal structure of TeNT-LC, determined by combined molecular replacement and MAD phasing. As expected, the overall structure of TeNT-LC is similar to the other known CNT light chain structures, including a conserved thermolysin-like core inserted between structurally distinct amino- and carboxy-terminal regions. Differences between TeNT-LC and the other CNT light chains are mainly limited to surface features such as unique electrostatic potential profiles. An analysis of surface residue conservation reveals a pattern of relatively high variability matching the path of substrate binding around BoNT/A, possibly serving to accommodate the variations in different SNARE targets of the CNT group.
About this Structure
1Z7H is a Single protein structure of sequence from Clostridium tetani. Full crystallographic information is available from OCA.
Reference
2.3 A crystal structure of tetanus neurotoxin light chain., Breidenbach MA, Brunger AT, Biochemistry. 2005 May 24;44(20):7450-7. PMID:15895988 Page seeded by OCA on Sat May 3 17:16:30 2008
