1zdh
From Proteopedia
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[[Image:1zdh.gif|left|200px]] | [[Image:1zdh.gif|left|200px]] | ||
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'''MS2 COAT PROTEIN/RNA COMPLEX''' | '''MS2 COAT PROTEIN/RNA COMPLEX''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: K Valegard,S Van Den Worm,L Liljas]] | [[Category: K Valegard,S Van Den Worm,L Liljas]] | ||
| - | [[Category: | + | [[Category: Coat protein]] |
| - | [[Category: | + | [[Category: Icosahedral virus]] |
| - | + | [[Category: Rna fragment]] | |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Viral protein capsid]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:40 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:29, 3 May 2008
MS2 COAT PROTEIN/RNA COMPLEX
Overview
Crystal structures of two complexes between recombinant MS2 capsids and RNA operator fragments have been determined at 2.7 A resolution. The coat protein of the RNA bacteriophage MS2 is bifunctional; it forms the icosahedral virus shell to protect the viral nucleic acid and it acts as a translational repressor by binding with high specificity to a unique site on the RNA, a single stem-loop structure, containing the initiation codon of the gene for the viral replicase. In order to determine the structure of these protein-RNA complexes, we have used chemically synthesized variants of the stem-loop fragment and soaked them into crystals of recombinant capsids. The RNA stem-loop, as bound to the protein, forms a crescent-like structure and interacts with the surface of the beta-sheet of a coat protein dimer. It makes protein contacts with seven phosphate groups on the 5' side of the stem-loop, with a pyrimidine base at position -5, which stacks onto a tyrosine, and with two exposed adenine bases, one in the loop and one at a bulge in the stem. Replacement of the wild-type uridine with a cytosine at position -5 increases the affinity of the RNA to the dimer significantly. The complex with RNA stem-loop having cytosine at this position differs from that of the wild-type complex mainly by having one extra intramolecular RNA interaction and one extra water-mediated hydrogen bond.
About this Structure
1ZDH is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
Reference
The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions., Valegard K, Murray JB, Stonehouse NJ, van den Worm S, Stockley PG, Liljas L, J Mol Biol. 1997 Aug 1;270(5):724-38. PMID:9245600 Page seeded by OCA on Sat May 3 17:29:40 2008
