1zdk
From Proteopedia
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'''STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX''' | '''STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX''' | ||
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[[Category: Valegard, K.]] | [[Category: Valegard, K.]] | ||
[[Category: Vandenworm, S.]] | [[Category: Vandenworm, S.]] | ||
| - | [[Category: | + | [[Category: Coat protein]] |
| - | [[Category: | + | [[Category: Icosahedral virus]] |
| - | + | [[Category: Rna fragment]] | |
| - | [[Category: | + | [[Category: Rna-binding]] |
| - | [[Category: | + | [[Category: Viral protein capsid]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:29:52 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:29, 3 May 2008
STRUCTURE OF BACTERIOPHAGE COAT PROTEIN-LOOP RNA COMPLEX
Overview
The coat protein of bacteriophage MS2 is known to bind specifically to an RNA hairpin formed within the MS2 genome. Structurally this hairpin is built up by an RNA double helix interrupted by one unpaired nucleotide and closed by a four-nucleotide loop. We have performed crystallographic studies of complexes between MS2 coat protein capsids and four RNA hairpin variants in order to evaluate the minimal requirements for tight binding to the coat protein and to obtain more information about the three-dimensional structure of these hairpins. An RNA fragment including the four loop nucleotides and a two-base-pair stem but without the unpaired nucleotide is sufficient for binding to the coat protein shell under the conditions used in this study. In contrast, an RNA fragment containing a stem with the unpaired nucleotide but missing the loop nucleotides does not bind to the protein shell.
About this Structure
1ZDK is a Single protein structure of sequence from Enterobacterio phage ms2. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: implications for binding requirements., Grahn E, Stonehouse NJ, Murray JB, van den Worm S, Valegard K, Fridborg K, Stockley PG, Liljas L, RNA. 1999 Jan;5(1):131-8. PMID:9917072 Page seeded by OCA on Sat May 3 17:29:52 2008
