1zdu
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1zdu.gif|left|200px]] | [[Image:1zdu.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1zdu", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_1zdu| PDB=1zdu | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''The Crystal Structure of Human Liver Receptor Homologue-1''' | '''The Crystal Structure of Human Liver Receptor Homologue-1''' | ||
| Line 38: | Line 35: | ||
[[Category: West, B L.]] | [[Category: West, B L.]] | ||
[[Category: Zhang, C.]] | [[Category: Zhang, C.]] | ||
| - | [[Category: | + | [[Category: Liver receptor homologue-1]] |
| - | [[Category: | + | [[Category: Lrh-1]] |
| - | [[Category: | + | [[Category: Nuclear receptor]] |
| - | [[Category: | + | [[Category: Phosphatidylethanolamine]] |
| - | [[Category: | + | [[Category: Phospholipid]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:30:26 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:30, 3 May 2008
The Crystal Structure of Human Liver Receptor Homologue-1
Overview
Steroidogenic factor-1 (SF-1) and liver receptor homologue-1 (LRH-1) belong to the fushi tarazu factor 1 subfamily of nuclear receptors. SF-1 is an essential factor for sex determination during development and regulates adrenal and gonadal steroidogenesis in the adult, whereas LRH-1 is a critical factor for development of endodermal tissues and regulates cholesterol and bile acid homeostasis. Regulatory ligands are unknown for SF-1 and LRH-1. A reported mouse LRH-1 structure revealed an empty pocket in a region commonly occupied by ligands in the structures of other nuclear receptors, and pocket-filling mutations did not alter the constitutive activity observed. Here we report the crystal structures of the putative ligand-binding domains of human SF-1 at 2.1-A resolution and human LRH-1 at 2.5-A resolution. Both structures bind a coactivator-derived peptide at the canonical activation-function surface, thus adopting the transcriptionally activating conformation. In human LRH-1, coactivator peptide binding also occurs to a second site. We discovered in both structures a phospholipid molecule bound in a pocket of the putative ligand-binding domain. MS analysis of the protein samples used for crystallization indicated that the two proteins associate with a range of phospholipids. Mutations of the pocket-lining residues reduced the transcriptional activities of SF-1 and LRH-1 in mammalian cell transfection assays without affecting their expression levels. These results suggest that human SF-1 and LRH-1 may be ligand-binding receptors, although it remains to be seen if phospholipids or possibly other molecules regulate SF-1 or LRH-1 under physiological conditions.
About this Structure
1ZDU is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The crystal structures of human steroidogenic factor-1 and liver receptor homologue-1., Wang W, Zhang C, Marimuthu A, Krupka HI, Tabrizizad M, Shelloe R, Mehra U, Eng K, Nguyen H, Settachatgul C, Powell B, Milburn MV, West BL, Proc Natl Acad Sci U S A. 2005 May 24;102(21):7505-10. Epub 2005 May 16. PMID:15897460 Page seeded by OCA on Sat May 3 17:30:26 2008
Categories: Homo sapiens | Protein complex | Eng, K. | Krupka, H I. | Marimuthu, A. | Mehra, U. | Milburn, M V. | Nguyen, H. | Powell, B. | Settachatgul, C. | Shelloe, R. | Tabrizizad, M. | Wang, W. | West, B L. | Zhang, C. | Liver receptor homologue-1 | Lrh-1 | Nuclear receptor | Phosphatidylethanolamine | Phospholipid
