1zgw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1zgw.gif|left|200px]]
[[Image:1zgw.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1zgw |SIZE=350|CAPTION= <scene name='initialview01'>1zgw</scene>
+
The line below this paragraph, containing "STRUCTURE_1zgw", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= ADA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1zgw| PDB=1zgw | SCENE= }}
-
|RELATEDENTRY=[[1u8b|1U8B]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zgw OCA], [http://www.ebi.ac.uk/pdbsum/1zgw PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zgw RCSB]</span>
+
-
}}
+
'''NMR structure of E. Coli Ada protein in complex with DNA'''
'''NMR structure of E. Coli Ada protein in complex with DNA'''
Line 36: Line 33:
[[Category: Wei, H.]]
[[Category: Wei, H.]]
[[Category: Zhou, P.]]
[[Category: Zhou, P.]]
-
[[Category: helix-turn-helix]]
+
[[Category: Helix-turn-helix]]
-
[[Category: protein-dna complex]]
+
[[Category: Protein-dna complex]]
-
[[Category: zinc ligand]]
+
[[Category: Zinc ligand]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:36:34 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:36:21 2008''
+

Revision as of 14:36, 3 May 2008

Image:1zgw.gif

Template:STRUCTURE 1zgw

NMR structure of E. Coli Ada protein in complex with DNA


Overview

The transcriptional activity of many sequence-specific DNA binding proteins is directly regulated by posttranslational covalent modification. Although this form of regulation was first described nearly two decades ago, it remains poorly understood at a mechanistic level. The prototype for a transcription factor controlled by posttranslational modification is E. coli Ada protein, a chemosensor that both repairs methylation damage in DNA and coordinates the resistance response to genotoxic methylating agents. Ada repairs methyl phosphotriester lesions in DNA by transferring the aberrant methyl group to one of its own cysteine residues; this site-specific methylation enhances tremendously the DNA binding activity of the protein, thereby enabling it to activate a methylation-resistance regulon. Here, we report solution and X-ray structures of the Cys-methylated chemosensor domain of Ada bound to DNA. The structures reveal that both phosphotriester repair and methylation-dependent transcriptional activation function through a zinc- and methylation-dependent electrostatic switch.

About this Structure

1ZGW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A methylation-dependent electrostatic switch controls DNA repair and transcriptional activation by E. coli ada., He C, Hus JC, Sun LJ, Zhou P, Norman DP, Dotsch V, Wei H, Gross JD, Lane WS, Wagner G, Verdine GL, Mol Cell. 2005 Oct 7;20(1):117-29. PMID:16209950 Page seeded by OCA on Sat May 3 17:36:34 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zgw"
Personal tools