1zh0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1zh0.gif|left|200px]]
[[Image:1zh0.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1zh0 |SIZE=350|CAPTION= <scene name='initialview01'>1zh0</scene>, resolution 1.900&Aring;
+
The line below this paragraph, containing "STRUCTURE_1zh0", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=NAL:BETA-(2-NAPHTHYL)-ALANINE'>NAL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tyrosine--tRNA_ligase Tyrosine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.1 6.1.1.1] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1zh0| PDB=1zh0 | SCENE= }}
-
|RELATEDENTRY=[[1zh6|1ZH6]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zh0 OCA], [http://www.ebi.ac.uk/pdbsum/1zh0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zh0 RCSB]</span>
+
-
}}
+
'''Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetasein complex with L-3-(2-napthyl)alanine'''
'''Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetasein complex with L-3-(2-napthyl)alanine'''
Line 30: Line 27:
[[Category: Spraggon, G.]]
[[Category: Spraggon, G.]]
[[Category: Turner, J M.]]
[[Category: Turner, J M.]]
-
[[Category: npala]]
+
[[Category: Npala]]
-
[[Category: structural plasticity]]
+
[[Category: Structural plasticity]]
-
[[Category: trna synthetase]]
+
[[Category: Trna synthetase]]
-
[[Category: unnatural amino acid]]
+
[[Category: Unnatural amino acid]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:36:49 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:36:19 2008''
+

Revision as of 14:36, 3 May 2008

Image:1zh0.gif

Template:STRUCTURE 1zh0

Crystal Structure of L-3-(2-napthyl)alanine-tRNA synthetasein complex with L-3-(2-napthyl)alanine


Overview

Recently, tRNA aminoacyl-tRNA synthetase pairs have been evolved that allow one to genetically encode a large array of unnatural amino acids in both prokaryotic and eukaryotic organisms. We have determined the crystal structures of two substrate-bound Methanococcus jannaschii tyrosyl aminoacyl-tRNA synthetases that charge the unnatural amino acids p-bromophenylalanine and 3-(2-naphthyl)alanine (NpAla). A comparison of these structures with the substrate-bound WT synthetase, as well as a mutant synthetase that charges p-acetylphenylalanine, shows that altered specificity is due to both side-chain and backbone rearrangements within the active site that modify hydrogen bonds and packing interactions with substrate, as well as disrupt the alpha8-helix, which spans the WT active site. The high degree of structural plasticity that is observed in these aminoacyl-tRNA synthetases is rarely found in other mutant enzymes with altered specificities and provides an explanation for the surprising adaptability of the genetic code to novel amino acids.

About this Structure

1ZH0 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

Reference

Structural plasticity of an aminoacyl-tRNA synthetase active site., Turner JM, Graziano J, Spraggon G, Schultz PG, Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6483-8. Epub 2006 Apr 17. PMID:16618920 Page seeded by OCA on Sat May 3 17:36:49 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1zh0"
Personal tools