This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1zjk
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1zjk.gif|left|200px]] | [[Image:1zjk.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1zjk", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1zjk| PDB=1zjk | SCENE= }} | |
| - | + | ||
| - | | | + | |
| - | }} | + | |
'''Crystal structure of the zymogen catalytic region of human MASP-2''' | '''Crystal structure of the zymogen catalytic region of human MASP-2''' | ||
| Line 39: | Line 36: | ||
[[Category: Vegh, B.]] | [[Category: Vegh, B.]] | ||
[[Category: Zavodszky, P.]] | [[Category: Zavodszky, P.]] | ||
| - | [[Category: | + | [[Category: Beta barrel]] |
| - | [[Category: | + | [[Category: Modular protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:42:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:42, 3 May 2008
Crystal structure of the zymogen catalytic region of human MASP-2
Contents |
Overview
Few reports have described in detail a true autoactivation process, where no extrinsic cleavage factors are required to initiate the autoactivation of a zymogen. Herein, we provide structural and mechanistic insight into the autoactivation of a multidomain serine protease: mannose-binding lectin-associated serine protease-2 (MASP-2), the first enzymatic component in the lectin pathway of complement activation. We characterized the proenzyme form of a MASP-2 catalytic fragment encompassing its C-terminal three domains and solved its crystal structure at 2.4 A resolution. Surprisingly, zymogen MASP-2 is capable of cleaving its natural substrate C4, with an efficiency about 10% that of active MASP-2. Comparison of the zymogen and active structures of MASP-2 reveals that, in addition to the activation domain, other loops of the serine protease domain undergo significant conformational changes. This additional flexibility could play a key role in the transition of zymogen MASP-2 into a proteolytically active form. Based on the three-dimensional structures of proenzyme and active MASP-2 catalytic fragments, we present model for the active zymogen MASP-2 complex and propose a mechanism for the autoactivation process.
Disease
Known disease associated with this structure: MASP2 deficiency OMIM:[605102]
About this Structure
1ZJK is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A true autoactivating enzyme. Structural insight into mannose-binding lectin-associated serine protease-2 activations., Gal P, Harmat V, Kocsis A, Bian T, Barna L, Ambrus G, Vegh B, Balczer J, Sim RB, Naray-Szabo G, Zavodszky P, J Biol Chem. 2005 Sep 30;280(39):33435-44. Epub 2005 Jul 21. PMID:16040602 Page seeded by OCA on Sat May 3 17:42:21 2008
Categories: Homo sapiens | Single protein | Ambrus, G. | Balczer, J. | Barna, L. | Bian, T. | Gal, P. | Harmat, V. | Kocsis, A. | Naray-Szabo, G. | Sim, R B. | Vegh, B. | Zavodszky, P. | Beta barrel | Modular protein
