2d10
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="2d10" size="450" color="white" frame="true" align="right" spinBox="true" caption="2d10, resolution 2.50Å" /> '''Crystal structure o...)
Next diff →
Revision as of 19:18, 12 November 2007
|
Crystal structure of the Radixin FERM domain complexed with the NHERF-1 C-terminal tail peptide
Overview
The Na+/H+ exchanger regulatory factor (NHERF) is a key adaptor protein, involved in the anchoring of ion channels and receptors to the actin, cytoskeleton through binding to ERM (ezrin/radixin/moesin) proteins. NHERF, binds the FERM domain of ERM proteins, although NHERF has no signature, Motif-1 sequence for FERM binding found in adhesion molecules. The crystal, structures of the radixin FERM domain complexed with the NHERF-1 and, NHERF-2 C-terminal peptides revealed a peptide binding site of the FERM, domain specific for the 13 residue motif MDWxxxxx(L/I)Fxx(L/F) (Motif-2), which is distinct from Motif-1. This Motif-2 forms an amphipathic alpha, helix for hydrophobic docking to subdomain C of the FERM domain. This, docking causes induced-fit conformational changes in subdomain C and, affects binding to adhesion molecule peptides, while the two binding sites, are not overlapped. Our studies provide structural paradigms for versatile, ERM linkages between membrane proteins and the cytoskeleton.
About this Structure
2D10 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for NHERF recognition by ERM proteins., Terawaki S, Maesaki R, Hakoshima T, Structure. 2006 Apr;14(4):777-89. PMID:16615918
Page seeded by OCA on Mon Nov 12 21:25:12 2007
