1zm2

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[[Image:1zm2.gif|left|200px]]
[[Image:1zm2.gif|left|200px]]
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{{Structure
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|PDB= 1zm2 |SIZE=350|CAPTION= <scene name='initialview01'>1zm2</scene>, resolution 3.07&Aring;
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The line below this paragraph, containing "STRUCTURE_1zm2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|GENE= eta ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
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|DOMAIN=
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{{STRUCTURE_1zm2| PDB=1zm2 | SCENE= }}
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|RELATEDENTRY=[[1u2r|1U2R]], [[1aer|1AER]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zm2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zm2 OCA], [http://www.ebi.ac.uk/pdbsum/1zm2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zm2 RCSB]</span>
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'''Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA'''
'''Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA'''
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[[Category: Schwan, A L.]]
[[Category: Schwan, A L.]]
[[Category: Yates, S P.]]
[[Category: Yates, S P.]]
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[[Category: adp-ribosylation]]
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[[Category: Adp-ribosylation]]
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[[Category: elongation factor]]
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[[Category: Elongation factor]]
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[[Category: toxin]]
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[[Category: Toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:47:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:38:31 2008''
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Revision as of 14:47, 3 May 2008

Image:1zm2.gif

Template:STRUCTURE 1zm2

Structure of ADP-ribosylated eEF2 in complex with catalytic fragment of ETA


Overview

The bacteria causing diphtheria, whooping cough, cholera and other diseases secrete mono-ADP-ribosylating toxins that modify intracellular proteins. Here, we describe four structures of a catalytically active complex between a fragment of Pseudomonas aeruginosa exotoxin A (ETA) and its protein substrate, translation elongation factor 2 (eEF2). The target residue in eEF2, diphthamide (a modified histidine), spans across a cleft and faces the two phosphates and a ribose of the non-hydrolysable NAD+ analogue, betaTAD. This suggests that the diphthamide is involved in triggering NAD+ cleavage and interacting with the proposed oxacarbenium intermediate during the nucleophilic substitution reaction, explaining the requirement of diphthamide for ADP ribosylation. Diphtheria toxin may recognize eEF2 in a manner similar to ETA. Notably, the toxin-bound betaTAD phosphates mimic the phosphate backbone of two nucleotides in a conformational switch of 18S rRNA, thereby achieving universal recognition of eEF2 by ETA.

About this Structure

1ZM2 is a Protein complex structure of sequences from Pseudomonas aeruginosa and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Exotoxin A-eEF2 complex structure indicates ADP ribosylation by ribosome mimicry., Jorgensen R, Merrill AR, Yates SP, Marquez VE, Schwan AL, Boesen T, Andersen GR, Nature. 2005 Aug 18;436(7053):979-84. PMID:16107839 Page seeded by OCA on Sat May 3 17:47:35 2008

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