2d1x

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Revision as of 19:19, 12 November 2007

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spinqualitylabelsall models 2d1x, resolution 1.90Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The crystal structure of the cortactin-SH3 domain and AMAP1-peptide complex

Overview

Invasive potentials of carcinomas greatly contribute to their metastasis, which is a major threat in most cancers. We have recently shown that Arf6, plays a pivotal role in breast cancer invasive activities and identified, AMAP1 as an effector of GTP-Arf6 in invasion. Expression of AMAP1, correlates well with invasive phenotypes of primary tumors of the human, breast. We also have shown that AMAP1 functions by forming a trimeric, protein complex with cortactin and paxillin. In this complex, AMAP1 binds, to the src homology 3 (SH3) domain of cortactin via its proline-rich, peptide, SKKRPPPPPPGHKRT. SH3 domains are known to bind generally to the, proline-rich ligands with a one-to-one stoichiometry. We found that, AMAP1/cortactin binding is very atypical in its stoichiometry and, interface structure, in which one AMAP1 proline-rich peptide binds to two, cortactin SH3 domains simultaneously. We made a cell-permeable peptide, derived from the AMAP1 peptide, and we show that this peptide specifically, blocks AMAP1/cortactin binding, but not other canonical SH3/proline, bindings, and effectively inhibits breast cancer invasion and metastasis., Moreover, this peptide was found to block invasion of other types of, cancers, such as glioblastomas and lung carcinomas. We also found that a, small-molecule compound, UCS15A, which was previously judged as a weak, inhibitor against canonical SH3/proline bindings, effectively inhibits, AMAP1/cortactin binding and breast cancer invasion and metastasis., Together with fine structural analysis, we propose that the, AMAP1/cortactin complex, which is not detected in normal mammary, epithelial cells, is an excellent drug target for cancer therapeutics.

About this Structure

2D1X is a Protein complex structure of sequences from Homo sapiens with SO4 as ligand. Full crystallographic information is available from OCA.

Reference

Targeting AMAP1 and cortactin binding bearing an atypical src homology 3/proline interface for prevention of breast cancer invasion and metastasis., Hashimoto S, Hirose M, Hashimoto A, Morishige M, Yamada A, Hosaka H, Akagi K, Ogawa E, Oneyama C, Agatsuma T, Okada M, Kobayashi H, Wada H, Nakano H, Ikegami T, Nakagawa A, Sabe H, Proc Natl Acad Sci U S A. 2006 May 2;103(18):7036-41. Epub 2006 Apr 24. PMID:16636290

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