1zmo
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1zmo.gif|left|200px]] | [[Image:1zmo.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1zmo", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1zmo| PDB=1zmo | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2''' | '''Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2''' | ||
| Line 30: | Line 27: | ||
[[Category: Kalk, K H.]] | [[Category: Kalk, K H.]] | ||
[[Category: Tang, L.]] | [[Category: Tang, L.]] | ||
| - | [[Category: | + | [[Category: Haloalcohol dehalogenase]] |
| - | [[Category: | + | [[Category: Halohydrin dehalogenase]] |
| - | [[Category: | + | [[Category: Short-chain dehydrogenase/reductase family]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:48:57 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:48, 3 May 2008
Apo structure of haloalcohol dehalogenase HheA of Arthrobacter sp. AD2
Overview
Haloalcohol dehalogenases are bacterial enzymes that cleave the carbon-halogen bond in short aliphatic vicinal haloalcohols, like 1-chloro-2,3-propanediol, some of which are recalcitrant environmental pollutants. They use a conserved Ser-Tyr-Arg catalytic triad to deprotonate the haloalcohol oxygen, which attacks the halogen-bearing carbon atom, producing an epoxide and a halide ion. Here, we present the X-ray structure of the haloalcohol dehalogenase HheA(AD2) from Arthrobacter sp. strain AD2 at 2.0-A resolution. Comparison with the previously reported structure of the 34% identical enantioselective haloalcohol dehalogenase HheC from Agrobacterium radiobacter AD1 shows that HheA(AD2) has a similar quaternary and tertiary structure but a much more open substrate-binding pocket. Docking experiments reveal that HheA(AD2) can bind both enantiomers of the haloalcohol substrate 1-p-nitrophenyl-2-chloroethanol in a productive way, which explains the low enantiopreference of HheA(AD2). Other differences are found in the halide-binding site, where the side chain amino group of Asn182 is in a position to stabilize the halogen atom or halide ion in HheA(AD2), in contrast to HheC, where a water molecule has taken over this role. These results broaden the insight into the structural determinants that govern reactivity and selectivity in the haloalcohol dehalogenase family.
About this Structure
1ZMO is a Single protein structure of sequence from Arthrobacter sp. ad2. Full crystallographic information is available from OCA.
Reference
The X-ray structure of the haloalcohol dehalogenase HheA from Arthrobacter sp. strain AD2: insight into enantioselectivity and halide binding in the haloalcohol dehalogenase family., de Jong RM, Kalk KH, Tang L, Janssen DB, Dijkstra BW, J Bacteriol. 2006 Jun;188(11):4051-6. PMID:16707696 Page seeded by OCA on Sat May 3 17:48:57 2008
