1zo3
From Proteopedia
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[[Image:1zo3.gif|left|200px]] | [[Image:1zo3.gif|left|200px]] | ||
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'''The P-site and P/E-site tRNA structures fitted to P/I site codon.''' | '''The P-site and P/E-site tRNA structures fitted to P/I site codon.''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZO3 OCA]. | |
==Reference== | ==Reference== | ||
The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15935757 15935757] | The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15935757 15935757] | ||
| - | [[Category: Escherichia coli]] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Allen, G S.]] | [[Category: Allen, G S.]] | ||
[[Category: Ehrenberg, M.]] | [[Category: Ehrenberg, M.]] | ||
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[[Category: Gursky, R.]] | [[Category: Gursky, R.]] | ||
[[Category: Zavialov, A.]] | [[Category: Zavialov, A.]] | ||
| - | [[Category: | + | [[Category: Cryo-eletron microscopy]] |
| - | [[Category: | + | [[Category: E. coli]] |
| - | [[Category: | + | [[Category: Initiation factor]] |
| - | [[Category: | + | [[Category: Initiation of protein synthesis]] |
| - | [[Category: | + | [[Category: Ribosome]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:03 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:52, 3 May 2008
The P-site and P/E-site tRNA structures fitted to P/I site codon.
Overview
The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.
About this Structure
Full crystallographic information is available from OCA.
Reference
The cryo-EM structure of a translation initiation complex from Escherichia coli., Allen GS, Zavialov A, Gursky R, Ehrenberg M, Frank J, Cell. 2005 Jun 3;121(5):703-12. PMID:15935757 Page seeded by OCA on Sat May 3 17:52:03 2008
