1zoi
From Proteopedia
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'''Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996''' | '''Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996''' | ||
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[[Category: Shaw, S Y.]] | [[Category: Shaw, S Y.]] | ||
[[Category: Wang, Y L.]] | [[Category: Wang, Y L.]] | ||
| - | [[Category: | + | [[Category: Alpha/beta hydrolase fold]] |
| - | [[Category: | + | [[Category: Esterase]] |
| - | [[Category: | + | [[Category: Pseudomonas putida]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 17:52:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 14:52, 3 May 2008
Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996
Overview
Esterase (EST) from Pseudomonas putida IFO12996 catalyzes the stereoselective hydrolysis of methyl dl-beta-acetylthioisobutyrate (dl-MATI) to produce d-beta-acetylthioisobutyric acid (DAT), serving as a key intermediate for the synthesis of angiotensin-converting enzyme inhibitors. The EST gene was cloned and expressed in Escherichia coli; the recombinant protein is a non-disulfide-linked homotrimer with a monomer molecular weight of 33,000 in both solution and crystalline states, indicating that these ESTs function as trimers. EST hydrolyzed dl-MATI to produce DAT with a degree of conversion of 49.5% and an enantiomeric excess value of 97.2% at an optimum pH of about 8 to 10 and an optimum temperature of about 57 to 67 degrees C. The crystal structure of EST has been determined by X-ray diffraction to a resolution of 1.6 A, confirming that EST is a member of the alpha/beta hydrolase fold superfamily of enzymes and includes a catalytic triad of Ser97, Asp227, and His256. The active site is located approximately in the middle of the molecule at the end of a pocket approximately 12 A deep. EST can hydrolyze the methyl ester group without affecting the acetylthiol ester moiety in dl-MATI. The examination of substrate specificity of EST toward other linear esters revealed that the enzyme showed specific activity toward methyl esters and that it recognized the configuration at C-2.
About this Structure
1ZOI is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.
Reference
Stereoselective esterase from Pseudomonas putida IFO12996 reveals alpha/beta hydrolase folds for D-beta-acetylthioisobutyric acid synthesis., Elmi F, Lee HT, Huang JY, Hsieh YC, Wang YL, Chen YJ, Shaw SY, Chen CJ, J Bacteriol. 2005 Dec;187(24):8470-6. PMID:16321951 Page seeded by OCA on Sat May 3 17:52:45 2008
