1zuh
From Proteopedia
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'''Structural Basis for Shikimate-binding Specificity of Helicobacter pylori Shikimate Kinase''' | '''Structural Basis for Shikimate-binding Specificity of Helicobacter pylori Shikimate Kinase''' | ||
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[[Category: Cheng, W C.]] | [[Category: Cheng, W C.]] | ||
[[Category: Wang, W C.]] | [[Category: Wang, W C.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:05:19 2008'' | |
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Revision as of 15:05, 3 May 2008
Structural Basis for Shikimate-binding Specificity of Helicobacter pylori Shikimate Kinase
Overview
Shikimate kinase (EC 2.7.1.71) catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid in the presence of ATP. As the fifth key step in the shikimate pathway for aromatic amino acid biosynthesis in bacteria, fungi, and plants, but not mammals, shikimate kinase represents an attractive target for the development of new antimicrobial agents, herbicides, and antiparasitic agents. Here, we report the 1.8-Angstroms crystal structure of Helicobacter pylori shikimate kinase (HpSK). The crystal structure shows a three-layer alpha/beta fold consisting of a central sheet of five parallel beta-strands flanked by seven alpha-helices. An HpSK-shikimate-PO(4) complex was also determined and refined to 2.3 Angstroms, revealing induced-fit movement from an open to a closed form on substrate binding. Shikimate is located above a short 3(10) helix formed by a strictly conserved motif (GGGXV) after beta(3). Moreover, several highly conserved charged residues including Asp33 (in a conserved DT/SD motif), Arg57, and Arg132 (interacting with shikimate) are identified, guiding the development of novel inhibitors of shikimate kinase.
About this Structure
1ZUH is a Single protein structure of sequence from Helicobacter pylori. Full crystallographic information is available from OCA.
Reference
Structural basis for shikimate-binding specificity of Helicobacter pylori shikimate kinase., Cheng WC, Chang YN, Wang WC, J Bacteriol. 2005 Dec;187(23):8156-63. PMID:16291688 Page seeded by OCA on Sat May 3 18:05:19 2008
