1zun

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[[Image:1zun.gif|left|200px]]
[[Image:1zun.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1zun |SIZE=350|CAPTION= <scene name='initialview01'>1zun</scene>, resolution 2.70&Aring;
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The line below this paragraph, containing "STRUCTURE_1zun", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Sulfate_adenylyltransferase Sulfate adenylyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.4 2.7.7.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= cysD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=317 Pseudomonas syringae]), cysNC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=223283 Pseudomonas syringae pv. tomato str. DC3000])
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-->
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|DOMAIN=
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{{STRUCTURE_1zun| PDB=1zun | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zun OCA], [http://www.ebi.ac.uk/pdbsum/1zun PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zun RCSB]</span>
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}}
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'''Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae'''
'''Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae'''
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[[Category: Schelle, M W.]]
[[Category: Schelle, M W.]]
[[Category: Vocadlo, D J.]]
[[Category: Vocadlo, D J.]]
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[[Category: beta barrel]]
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[[Category: Beta barrel]]
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[[Category: g protein]]
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[[Category: G protein]]
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[[Category: gtpase]]
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[[Category: Gtpase]]
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[[Category: heterodimer]]
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[[Category: Heterodimer]]
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[[Category: pyrophosphate]]
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[[Category: Pyrophosphate]]
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[[Category: switch domain]]
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[[Category: Switch domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:05:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:41:49 2008''
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Revision as of 15:05, 3 May 2008

Image:1zun.gif

Template:STRUCTURE 1zun

Crystal Structure of a GTP-Regulated ATP Sulfurylase Heterodimer from Pseudomonas syringae


Overview

Sulfate assimilation is a critical component of both primary and secondary metabolism. An essential step in this pathway is the activation of sulfate through adenylation by the enzyme ATP sulfurylase (ATPS), forming adenosine 5'-phosphosulfate (APS). Proteobacterial ATPS overcomes this energetically unfavorable reaction by associating with a regulatory G protein, coupling the energy of GTP hydrolysis to APS formation. To discover the molecular basis of this unusual role for a G protein, we biochemically characterized and solved the X-ray crystal structure of a complex between Pseudomonas syringae ATPS (CysD) and its associated regulatory G protein (CysN). The structure of CysN*D shows the two proteins in tight association; however, the nucleotides bound to each subunit are spatially segregated. We provide evidence that conserved switch motifs in the G domain of CysN allosterically mediate interactions between the nucleotide binding sites. This structure suggests a molecular mechanism by which conserved G domain architecture is used to energetically link GTP turnover to the production of an essential metabolite.

About this Structure

1ZUN is a Protein complex structure of sequences from Pseudomonas syringae and Pseudomonas syringae pv. tomato str. dc3000. Full crystallographic information is available from OCA.

Reference

Molecular basis for G protein control of the prokaryotic ATP sulfurylase., Mougous JD, Lee DH, Hubbard SC, Schelle MW, Vocadlo DJ, Berger JM, Bertozzi CR, Mol Cell. 2006 Jan 6;21(1):109-22. PMID:16387658 Page seeded by OCA on Sat May 3 18:05:37 2008

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