1zvq

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[[Image:1zvq.gif|left|200px]]
[[Image:1zvq.gif|left|200px]]
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{{Structure
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|PDB= 1zvq |SIZE=350|CAPTION= <scene name='initialview01'>1zvq</scene>, resolution 2.0&Aring;
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The line below this paragraph, containing "STRUCTURE_1zvq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= HRAS, HRAS1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_1zvq| PDB=1zvq | SCENE= }}
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|RELATEDENTRY=[[4q21|4Q21]], [[1lf5|1LF5]], [[1zw6|1ZW6]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zvq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zvq OCA], [http://www.ebi.ac.uk/pdbsum/1zvq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zvq RCSB]</span>
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'''Structure of the Q61G mutant of Ras in the GDP-bound form'''
'''Structure of the Q61G mutant of Ras in the GDP-bound form'''
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[[Category: Ford, B.]]
[[Category: Ford, B.]]
[[Category: Nassar, N.]]
[[Category: Nassar, N.]]
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[[Category: gdp]]
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[[Category: Gdp]]
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[[Category: gtpase]]
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[[Category: Gtpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:08:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:42:18 2008''
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Revision as of 15:08, 3 May 2008

Image:1zvq.gif

Template:STRUCTURE 1zvq

Structure of the Q61G mutant of Ras in the GDP-bound form


Overview

The flexibility of the conserved 57DTAGQ61 motif is essential for Ras proper cycling in response to growth factors. Here, we increase the flexibility of the 57DTAGQ61 motif by mutating Gln61 to Gly. The crystal structure of the RasQ61G mutant reveals a new conformation of switch 2 that bears remarkable structural homology to an intermediate for GTP hydrolysis revealed by targeted molecular dynamics simulations. The mutation increased retention of GTP and inhibited Ras binding to the catalytic site, but not to the distal site of Sos. Most importantly, the thermodynamics of RafRBD binding to Ras are altered even though the structure of switch 1 is not affected by the mutation. Our results suggest that interplay and transmission of structural information between the switch regions are important factors for Ras function. They propose that initiation of GTP hydrolysis sets off the separation of the Ras/effector complex even before the GDP conformation is reached.

About this Structure

1ZVQ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structure of a transient intermediate for GTP hydrolysis by ras., Ford B, Hornak V, Kleinman H, Nassar N, Structure. 2006 Mar;14(3):427-36. PMID:16531227 Page seeded by OCA on Sat May 3 18:08:12 2008

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