1zyo

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[[Image:1zyo.gif|left|200px]]
[[Image:1zyo.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1zyo |SIZE=350|CAPTION= <scene name='initialview01'>1zyo</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1zyo", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamyl_endopeptidase Glutamyl endopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.19 3.4.21.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= ORF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12558 Sesbania mosaic virus])
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|DOMAIN=
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{{STRUCTURE_1zyo| PDB=1zyo | SCENE= }}
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|RELATEDENTRY=[[1lvm|1LVM]], [[1q31|1Q31]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyo OCA], [http://www.ebi.ac.uk/pdbsum/1zyo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zyo RCSB]</span>
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}}
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'''Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein'''
'''Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein'''
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[[Category: Satheshkumar, P S.]]
[[Category: Satheshkumar, P S.]]
[[Category: Savithri, H S.]]
[[Category: Savithri, H S.]]
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[[Category: beta-barrel]]
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[[Category: Beta-barrel]]
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[[Category: glutamyl endopeptidase]]
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[[Category: Glutamyl endopeptidase]]
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[[Category: viral serine protease of trypsin fold]]
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[[Category: Viral serine protease of trypsin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:14:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:22 2008''
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Revision as of 15:14, 3 May 2008

Image:1zyo.gif

Template:STRUCTURE 1zyo

Crystal Structure of the Serine Protease Domain of Sesbania Mosaic Virus polyprotein


Overview

Sesbania mosaic virus (SeMV) polyprotein is processed by its N-terminal serine protease domain. The crystal structure of the protease domain was determined to a resolution of 2.4 A using multiple isomorphous replacement and anomalous scattering. The SeMV protease domain exhibited the characteristic trypsin fold and was found to be closer to cellular serine proteases than to other viral proteases. The residues of the S1-binding pocket, H298, T279 and N308 were mutated to alanine in the DeltaN70-Protease-VPg polyprotein, and the cis-cleavage activity was examined. The H298A and T279A mutants were inactive, while the N308A mutant was partially active, suggesting that the interactions of H298 and T279 with P1-glutamate are crucial for the E-T/S cleavage. A region of exposed aromatic amino acids, probably essential for interaction with VPg, was identified on the protease domain, and this interaction could play a major role in modulating the function of the protease.

About this Structure

1ZYO is a Single protein structure of sequence from Sesbania mosaic virus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the serine protease domain of Sesbania mosaic virus polyprotein and mutational analysis of residues forming the S1-binding pocket., Gayathri P, Satheshkumar PS, Prasad K, Nair S, Savithri HS, Murthy MR, Virology. 2006 Mar 15;346(2):440-51. Epub 2005 Dec 13. PMID:16356524 Page seeded by OCA on Sat May 3 18:14:44 2008

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