226d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:226d.gif|left|200px]]
[[Image:226d.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 226d |SIZE=350|CAPTION= <scene name='initialview01'>226d</scene>
+
The line below this paragraph, containing "STRUCTURE_226d", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=BIZ:BIZELESIN'>BIZ</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_226d| PDB=226d | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=226d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=226d OCA], [http://www.ebi.ac.uk/pdbsum/226d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=226d RCSB]</span>
+
-
}}
+
'''SOLUTION CONFORMATION OF A BIZELESIN A-TRACT DUPLEX ADDUCT, NMR, 1 STRUCTURE'''
'''SOLUTION CONFORMATION OF A BIZELESIN A-TRACT DUPLEX ADDUCT, NMR, 1 STRUCTURE'''
Line 19: Line 16:
==About this Structure==
==About this Structure==
-
226D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=226D OCA].
+
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=226D OCA].
==Reference==
==Reference==
Solution conformation of a bizelesin A-tract duplex adduct: DNA-DNA cross-linking of an A-tract straightens out bent DNA., Thompson AS, Hurley LH, J Mol Biol. 1995 Sep 8;252(1):86-101. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7666436 7666436]
Solution conformation of a bizelesin A-tract duplex adduct: DNA-DNA cross-linking of an A-tract straightens out bent DNA., Thompson AS, Hurley LH, J Mol Biol. 1995 Sep 8;252(1):86-101. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7666436 7666436]
-
[[Category: Protein complex]]
 
[[Category: Hurley, L H.]]
[[Category: Hurley, L H.]]
[[Category: Thompson, A S.]]
[[Category: Thompson, A S.]]
-
[[Category: adduct]]
+
[[Category: Adduct]]
-
[[Category: bizelesin]]
+
[[Category: Bizelesin]]
-
[[Category: dna]]
+
[[Category: Dna]]
-
[[Category: dna-ligand]]
+
[[Category: Dna-ligand]]
-
[[Category: nmr]]
+
[[Category: Nmr]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:20:18 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:44:39 2008''
+

Revision as of 15:20, 3 May 2008

Image:226d.gif

Template:STRUCTURE 226d

SOLUTION CONFORMATION OF A BIZELESIN A-TRACT DUPLEX ADDUCT, NMR, 1 STRUCTURE


Overview

The DNA cross-linker bizelesin has been previously shown to form interhelical interstrand cross-links with adenine residues six base-pairs apart (including the modified adenine residues). Sequence specificity studies have shown that the ligand has a high affinity for the intrinsically bent A-tract sequence [d(CGTTTTTACG):d(CGTAAAAACG)]. However, gel retardation studies have shown that the cross-linked duplex retains none of the characteristic A-tract bending observed within the unmodified duplex. Two-dimensional 1H-NMR experiments have not only confirmed the sites of cross-linking into the duplex, but have also shown the loss of inherent A-tract characteristics, including reduced crosspeak intensities between the H2s of the central adenine residues and the cross-strand H1' of the base one base removed to the 3' side. This observation suggests loss of propeller twisting within these central adenine residues and provides insight into the controversial origin of A-tract bending. This study is important because it validates the use of bizelesin as a probe for determining the importance of A-tract-induced bending in transcriptional and replicational elements.

About this Structure

Full crystallographic information is available from OCA.

Reference

Solution conformation of a bizelesin A-tract duplex adduct: DNA-DNA cross-linking of an A-tract straightens out bent DNA., Thompson AS, Hurley LH, J Mol Biol. 1995 Sep 8;252(1):86-101. PMID:7666436 Page seeded by OCA on Sat May 3 18:20:18 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/226d"
Personal tools