233d

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[[Image:233d.gif|left|200px]]
[[Image:233d.gif|left|200px]]
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{{Structure
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|PDB= 233d |SIZE=350|CAPTION= <scene name='initialview01'>233d</scene>, resolution 2.400&Aring;
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The line below this paragraph, containing "STRUCTURE_233d", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=T49:S4&#39;-2&#39;DEOXYTHYMIDINE+5&#39;-MONOPHOSPHATE'>T49</scene>
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|DOMAIN=
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{{STRUCTURE_233d| PDB=233d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=233d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=233d OCA], [http://www.ebi.ac.uk/pdbsum/233d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=233d RCSB]</span>
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'''THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES'''
'''THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES'''
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==About this Structure==
==About this Structure==
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233D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=233D OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=233D OCA].
==Reference==
==Reference==
The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides., Boggon TJ, Hancox EL, McAuley-Hecht KE, Connolly BA, Hunter WN, Brown T, Walker RT, Leonard GA, Nucleic Acids Res. 1996 Mar 1;24(5):951-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8600465 8600465]
The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides., Boggon TJ, Hancox EL, McAuley-Hecht KE, Connolly BA, Hunter WN, Brown T, Walker RT, Leonard GA, Nucleic Acids Res. 1996 Mar 1;24(5):951-61. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8600465 8600465]
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[[Category: Protein complex]]
 
[[Category: Boggon, T J.]]
[[Category: Boggon, T J.]]
[[Category: Brown, T.]]
[[Category: Brown, T.]]
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[[Category: McAuley-Hecht, K E.]]
[[Category: McAuley-Hecht, K E.]]
[[Category: Walker, R T.]]
[[Category: Walker, R T.]]
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[[Category: b-dna]]
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[[Category: B-dna]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: modified]]
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[[Category: Modified]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:21:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:44:51 2008''
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Revision as of 15:21, 3 May 2008

Image:233d.gif

Template:STRUCTURE 233d

THE CRYSTAL STRUCTURE ANALYSIS OF D(CGCGAASSCGCG)2: A SYNTHETIC DNA DODECAMER DUPLEX CONTAINING FOUR 4'-THIO-2'-DEOXYTHYMIDINE NUCLEOTIDES


Overview

The crystal structure refinement of the synthetic dodecamer d(CGCGAASSCGCG), where S = 4'-thio-2'-deoxythymidine, has converged at R=0.201 for 2605 reflections with F > 2sigma(F) in the resolution range 8.0-2.4 A for a model consisting of the dodecamer duplex and 66 water molecules. A comparison of its structure with that of the native dodecamer d(CGCGAATTCGCG) has revealed that the major differences between the two structures is a change in the conformation of the sugar-phosphate backbone in the regions at and adjacent to the positions of the modified nucleosides. Examination of the fine structural parameters for each of the structures reveals that the thiosugars adopt a C3'-exo conformation in d(CGCGAASSCGCG), rather than the approximate C1'-exo conformation found for the analogous sugars in the structure of d(CGCGAATTCGCG). The observed differences in structure between the two duplexes may help to explain the enhanced resistance to nuclease digestion of synthetic oligonucleotides containing 4'-thio-2'-deoxynucleotides.

About this Structure

Full crystallographic information is available from OCA.

Reference

The crystal structure analysis of d(CGCGAASSCGCG)2, a synthetic DNA dodecamer duplex containing four 4'-thio-2'-deoxythymidine nucleotides., Boggon TJ, Hancox EL, McAuley-Hecht KE, Connolly BA, Hunter WN, Brown T, Walker RT, Leonard GA, Nucleic Acids Res. 1996 Mar 1;24(5):951-61. PMID:8600465 Page seeded by OCA on Sat May 3 18:21:13 2008

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