282d
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:282d.gif|left|200px]] | [[Image:282d.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_282d", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | | | + | or leave the SCENE parameter empty for the default display. |
| - | | | + | --> |
| - | + | {{STRUCTURE_282d| PDB=282d | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''A CONTINOUS TRANSITION FROM A-DNA TO B-DNA IN THE 1:1 COMPLEX BETWEEN NOGALAMYCIN AND THE HEXAMER DCCCGGG''' | '''A CONTINOUS TRANSITION FROM A-DNA TO B-DNA IN THE 1:1 COMPLEX BETWEEN NOGALAMYCIN AND THE HEXAMER DCCCGGG''' | ||
| Line 19: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=282D OCA]. | |
==Reference== | ==Reference== | ||
A continuous transition from A-DNA to B-DNA in the 1:1 complex between nogalamycin and the hexamer dCCCGGG., Cruse WB, Saludjian P, Leroux Y, Leger G, Manouni DE, Prange T, J Biol Chem. 1996 Jun 28;271(26):15558-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8662899 8662899] | A continuous transition from A-DNA to B-DNA in the 1:1 complex between nogalamycin and the hexamer dCCCGGG., Cruse WB, Saludjian P, Leroux Y, Leger G, Manouni DE, Prange T, J Biol Chem. 1996 Jun 28;271(26):15558-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8662899 8662899] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Cruse, W.]] | [[Category: Cruse, W.]] | ||
[[Category: Leger, Y.]] | [[Category: Leger, Y.]] | ||
| Line 30: | Line 26: | ||
[[Category: Prange, T.]] | [[Category: Prange, T.]] | ||
[[Category: Saludjian, P.]] | [[Category: Saludjian, P.]] | ||
| - | [[Category: | + | [[Category: Complexed with drug]] |
| - | [[Category: | + | [[Category: Double helix]] |
| - | [[Category: | + | [[Category: Right handed dna]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:25:17 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:25, 3 May 2008
A CONTINOUS TRANSITION FROM A-DNA TO B-DNA IN THE 1:1 COMPLEX BETWEEN NOGALAMYCIN AND THE HEXAMER DCCCGGG
Overview
The antibiotic nogalamycin, a drug with high specificity for TG and CG steps in double-stranded DNA, has been crystallized as a 1:1 complex with the hexamer d(CCCGGG). The antibiotic is inserted at the central CG step of the duplex, with the two sugars oriented in the same direction and with strong interactions with the DNA within the grooves. The amino-glucose residue makes an integral part of a well defined major groove hydration network with van der Waals contacts and several strong hydrogen bonds to the duplex. The nogalose residue resides in the minor groove, making primarily van der Waals contacts. The single site allows an accurate molecular description of the intercalation, without perturbations from end effects observed previously. The local unwinding induced by nogalamycin is completely relaxed 2 base pairs away from the intercalation site. The two strands of the DNA show a continuous deformation from the A to the B form: 1) the cytosines toward the 5' end of the nogalomycin site in each strand have c3'-endo conformations while 5 guanosines toward the 3' ends have c2'-endo conformations; 2) within each strand, the phosphate-phosphate distances increase in a continuous manner from 5.7 A (A-form) to 7.1 A (B-form).
About this Structure
Full crystallographic information is available from OCA.
Reference
A continuous transition from A-DNA to B-DNA in the 1:1 complex between nogalamycin and the hexamer dCCCGGG., Cruse WB, Saludjian P, Leroux Y, Leger G, Manouni DE, Prange T, J Biol Chem. 1996 Jun 28;271(26):15558-67. PMID:8662899 Page seeded by OCA on Sat May 3 18:25:17 2008
