283d

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[[Image:283d.jpg|left|200px]]
[[Image:283d.jpg|left|200px]]
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{{Structure
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|PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300&Aring;
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The line below this paragraph, containing "STRUCTURE_283d", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
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{{STRUCTURE_283d| PDB=283d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=283d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=283d OCA], [http://www.ebi.ac.uk/pdbsum/283d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=283d RCSB]</span>
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'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''
'''A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING'''
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==About this Structure==
==About this Structure==
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283D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=283D OCA].
==Reference==
==Reference==
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8917508 8917508]
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8917508 8917508]
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[[Category: ]]
 
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[[Category: Protein complex]]
 
[[Category: Baeyens, K J.]]
[[Category: Baeyens, K J.]]
[[Category: Bondt, H L.De.]]
[[Category: Bondt, H L.De.]]
[[Category: Holbrook, S R.]]
[[Category: Holbrook, S R.]]
[[Category: Pardi, A.]]
[[Category: Pardi, A.]]
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[[Category: curved]]
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[[Category: Curved]]
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[[Category: double helix]]
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[[Category: Double helix]]
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[[Category: internal loop]]
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[[Category: Internal loop]]
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[[Category: mismatched]]
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[[Category: Mismatched]]
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[[Category: unusual rna]]
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[[Category: Unusual rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:25:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:52 2008''
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Revision as of 15:25, 3 May 2008

Template:STRUCTURE 283d

A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING


Overview

The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.

About this Structure

Full crystallographic information is available from OCA.

Reference

A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 Page seeded by OCA on Sat May 3 18:25:19 2008

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