296d

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[[Image:296d.gif|left|200px]]
[[Image:296d.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 296d |SIZE=350|CAPTION= <scene name='initialview01'>296d</scene>, resolution 2.250&Aring;
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The line below this paragraph, containing "STRUCTURE_296d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=HT:2&#39;-(4-HYDROXYPHENYL)-5-(4-METHYL-1-PIPERAZINYL)-2,5&#39;-BI-BENZIMIDAZOLE'>HT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE=
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|DOMAIN=
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{{STRUCTURE_296d| PDB=296d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=296d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=296d OCA], [http://www.ebi.ac.uk/pdbsum/296d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=296d RCSB]</span>
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'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''
'''SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX'''
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==About this Structure==
==About this Structure==
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296D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=296D OCA].
==Reference==
==Reference==
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488]
Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7515488 7515488]
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[[Category: Protein complex]]
 
[[Category: Brown, D G.]]
[[Category: Brown, D G.]]
[[Category: Neidle, S.]]
[[Category: Neidle, S.]]
[[Category: Skelly, J V.]]
[[Category: Skelly, J V.]]
[[Category: Spink, N.]]
[[Category: Spink, N.]]
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[[Category: b-dna]]
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[[Category: B-dna]]
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[[Category: complexed with drug]]
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[[Category: Complexed with drug]]
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[[Category: double helix]]
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[[Category: Double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:26:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:01 2008''
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Revision as of 15:26, 3 May 2008

Image:296d.gif

Template:STRUCTURE 296d

SEQUENCE-DEPENDENT EFFECTS IN DRUG-DNA INTERACTION: THE CRYSTAL STRUCTURE OF HOECHST 33258 BOUND TO THE D(CGCAAATTTGCG)2 DUPLEX


Overview

The bis-benzimidazole drug Hoechst 33258 has been co-crystallized with the dodecanucleotide sequence d(CGCAAATTTGCG)2. The structure has been solved by molecular replacement and refined to an R factor of 18.5% for 2125 reflections collected on a Xentronics area detector. The drug is bound in the minor groove, at the five base-pair site 5'-ATTTG and is in a unique orientation. This is displaced by one base pair in the 5' direction compared to previously-determined structures of this drug with the sequence d(CGCGAATTCGCG)2. Reasons for this difference in behaviour are discussed in terms of several sequence-dependent structural features of the DNA, with particular reference to differences in propeller twist and minor-groove width.

About this Structure

Full crystallographic information is available from OCA.

Reference

Sequence-dependent effects in drug-DNA interaction: the crystal structure of Hoechst 33258 bound to the d(CGCAAATTTGCG)2 duplex., Spink N, Brown DG, Skelly JV, Neidle S, Nucleic Acids Res. 1994 May 11;22(9):1607-12. PMID:7515488 Page seeded by OCA on Sat May 3 18:26:08 2008

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