2dh3
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Revision as of 19:25, 12 November 2007
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Crystal Structure of human ED-4F2hc
Overview
4F2hc (CD98hc) is a multifunctional type II membrane glycoprotein involved, in amino acid transport and cell fusion, adhesion and transformation. The, structure of the ectodomain of human 4F2hc has been solved using, monoclinic (2DH2) and orthorhombic (2DH3) crystal forms at 2.1 and 2.8 A, respectively. It is composed of a (betaalpha)(8) barrel and an, antiparallel beta(8) sandwich related to bacterial alpha-glycosidases, although lacking key catalytic residues and consequently catalytic, activity. 2DH3 is a dimer with Zn(2+) coordination at the interface. Human, 4F2hc expressed in several cell types resulted in cell surface and Cys109, disulfide bridge-linked homodimers with major architecture features of the, crystal dimer, as demonstrated by cross-linking experiments. 4F2hc has no, significant hydrophobic patches at the surface. Monomer and homodimer have, a polarized charged surface. The N-terminus of the solved structure, including position of Cys109 residue located four residues apart from the, transmembrane domain, is adjacent to the positive face of the ectodomain., This location of the N-terminus and the Cys109-intervening disulfide, bridge imposes space restrictions sufficient to support a model for, electrostatic interaction of 4F2hc ectodomain with membrane phospholipids., These results provide the first crystal structure of Heteromeric Amino, acid Transporters and suggest a dynamic interaction of 4F2hc ectodomain, with plasma membrane.
About this Structure
2DH3 is a Single protein structure of sequence from Homo sapiens with ZN as ligand. Full crystallographic information is available from OCA.
Reference
The structure of human 4F2hc ectodomain provides a model for homodimerization and electrostatic interaction with plasma membrane., Fort J, de la Ballina LR, Burghardt HE, Ferrer-Costa C, Turnay J, Ferrer-Orta C, Uson I, Zorzano A, Fernandez-Recio J, Orozco M, Lizarbe MA, Fita I, Palacin M, J Biol Chem. 2007 Aug 26;. PMID:17724034
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