2a0j

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2a0j.gif|left|200px]]
[[Image:2a0j.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2a0j |SIZE=350|CAPTION= <scene name='initialview01'>2a0j</scene>, resolution 2.50&Aring;
+
The line below this paragraph, containing "STRUCTURE_2a0j", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE= ptsN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=487 Neisseria meningitidis])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2a0j| PDB=2a0j | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a0j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a0j OCA], [http://www.ebi.ac.uk/pdbsum/2a0j PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a0j RCSB]</span>
+
-
}}
+
'''Crystal Structure of Nitrogen Regulatory Protein IIA-Ntr from Neisseria meningitidis'''
'''Crystal Structure of Nitrogen Regulatory Protein IIA-Ntr from Neisseria meningitidis'''
Line 24: Line 21:
Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis., Ren J, Sainsbury S, Berrow NS, Alderton D, Nettleship JE, Stammers DK, Saunders NJ, Owens RJ, BMC Struct Biol. 2005 Aug 10;5:13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16092953 16092953]
Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis., Ren J, Sainsbury S, Berrow NS, Alderton D, Nettleship JE, Stammers DK, Saunders NJ, Owens RJ, BMC Struct Biol. 2005 Aug 10;5:13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16092953 16092953]
[[Category: Neisseria meningitidis]]
[[Category: Neisseria meningitidis]]
-
[[Category: Protein-N(pi)-phosphohistidine--sugar phosphotransferase]]
 
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Alderton, D.]]
[[Category: Alderton, D.]]
Line 35: Line 31:
[[Category: Saunders, N J.]]
[[Category: Saunders, N J.]]
[[Category: Stammers, D K.]]
[[Category: Stammers, D K.]]
-
[[Category: nitrogen regulation]]
+
[[Category: Nitrogen regulation]]
-
[[Category: oppf]]
+
[[Category: Oppf]]
-
[[Category: oxford protein production facility]]
+
[[Category: Oxford protein production facility]]
-
[[Category: structural genomic]]
+
[[Category: Structural genomic]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:27:15 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:14 2008''
+

Revision as of 15:27, 3 May 2008

Image:2a0j.gif

Template:STRUCTURE 2a0j

Crystal Structure of Nitrogen Regulatory Protein IIA-Ntr from Neisseria meningitidis


Overview

BACKGROUND: The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIANtr (abbreviated to NM-IIANtr). The homologous protein present in Escherichia coli is implicated in the control of nitrogen assimilation. As part of a structural proteomics approach to the study of pathogenic Neisseria spp., we have selected this protein for structure determination by X-ray crystallography. RESULTS: The NM-IIANtr was over-expressed in E. coli and was shown to be partially mono-phosphorylated, as assessed by mass spectrometry of the purified protein. Crystals of un-phosphorylated protein were obtained and diffraction data collected to 2.5 A resolution. The structure of NM-IIANtr was solved by molecular replacement using the coordinates of the E. coli nitrogen regulatory protein IIAntr [PDB: 1A6J] as the starting model. The overall fold of the Neisseria enzyme shows a high degree of similarity to the IIANtr from E. coli, and the position of the phosphoryl acceptor histidine residue (H67) is conserved. The orientation of an adjacent arginine residue (R69) suggests that it may also be involved in coordinating the phosphate group. Comparison of the structure with that of E. coli IIAmtl complexed with HPr [PDB: 1J6T] indicates that NM-IIANtr binds in a similar way to the HPr-like enzyme in Neisseria. CONCLUSION: The structure of NM-IIANtr confirms its assignment as a homologue of the IIANtr proteins found in a range of other Gram-negative bacteria. We conclude that the NM- IIANtr protein functions as part of a phosphorylation cascade which, in contrast to E. coli, shares the upstream phosphotransfer protein with the sugar uptake phosphoenolpyruvate:sugar phosphotransferase system (PTS), but in common with E. coli has a distinct downstream effector mechanism.

About this Structure

2A0J is a Single protein structure of sequence from Neisseria meningitidis. Full crystallographic information is available from OCA.

Reference

Crystal structure of nitrogen regulatory protein IIANtr from Neisseria meningitidis., Ren J, Sainsbury S, Berrow NS, Alderton D, Nettleship JE, Stammers DK, Saunders NJ, Owens RJ, BMC Struct Biol. 2005 Aug 10;5:13. PMID:16092953 Page seeded by OCA on Sat May 3 18:27:15 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a0j"
Personal tools