2a1t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2a1t.gif|left|200px]]
[[Image:2a1t.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2a1t |SIZE=350|CAPTION= <scene name='initialview01'>2a1t</scene>, resolution 2.80&Aring;
+
The line below this paragraph, containing "STRUCTURE_2a1t", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acyl-CoA_dehydrogenase Acyl-CoA dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.3 1.3.99.3] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2a1t| PDB=2a1t | SCENE= }}
-
|RELATEDENTRY=[[1t9g|1T9G]], [[2a1u|2A1U]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a1t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a1t OCA], [http://www.ebi.ac.uk/pdbsum/2a1t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a1t RCSB]</span>
+
-
}}
+
'''Structure of the human MCAD:ETF E165betaA complex'''
'''Structure of the human MCAD:ETF E165betaA complex'''
Line 30: Line 27:
[[Category: Thiel, A Van.]]
[[Category: Thiel, A Van.]]
[[Category: Toogood, H S.]]
[[Category: Toogood, H S.]]
-
[[Category: conformational sampling]]
+
[[Category: Conformational sampling]]
-
[[Category: domain dynamic]]
+
[[Category: Domain dynamic]]
-
[[Category: electron transfer]]
+
[[Category: Electron transfer]]
-
[[Category: fatty acid b-degradation]]
+
[[Category: Fatty acid b-degradation]]
-
[[Category: protein:protein complex]]
+
[[Category: Protein:protein complex]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:29:54 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:46:40 2008''
+

Revision as of 15:29, 3 May 2008

Image:2a1t.gif

Template:STRUCTURE 2a1t

Structure of the human MCAD:ETF E165betaA complex


Overview

Crystal structures of protein complexes with electron-transferring flavoprotein (ETF) have revealed a dual protein-protein interface with one region serving as anchor while the ETF FAD domain samples available space within the complex. We show that mutation of the conserved Glu-165beta in human ETF leads to drastically modulated rates of interprotein electron transfer with both medium chain acyl-CoA dehydrogenase and dimethylglycine dehydrogenase. The crystal structure of free E165betaA ETF is essentially identical to that of wild-type ETF, but the crystal structure of the E165betaA ETF.medium chain acyl-CoA dehydrogenase complex reveals clear electron density for the FAD domain in a position optimal for fast interprotein electron transfer. Based on our observations, we present a dynamic multistate model for conformational sampling that for the wild-type ETF. medium chain acyl-CoA dehydrogenase complex involves random motion between three distinct positions for the ETF FAD domain. ETF Glu-165beta plays a key role in stabilizing positions incompatible with fast interprotein electron transfer, thus ensuring high rates of complex dissociation.

About this Structure

2A1T is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Stabilization of non-productive conformations underpins rapid electron transfer to electron-transferring flavoprotein., Toogood HS, van Thiel A, Scrutton NS, Leys D, J Biol Chem. 2005 Aug 26;280(34):30361-6. Epub 2005 Jun 23. PMID:15975918 Page seeded by OCA on Sat May 3 18:29:54 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a1t"
Personal tools