2a3j
From Proteopedia
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[[Image:2a3j.gif|left|200px]] | [[Image:2a3j.gif|left|200px]] | ||
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'''Structure of URNdesign, a complete computational redesign of human U1A protein''' | '''Structure of URNdesign, a complete computational redesign of human U1A protein''' | ||
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[[Category: Dobson, N.]] | [[Category: Dobson, N.]] | ||
[[Category: Varani, G.]] | [[Category: Varani, G.]] | ||
| - | [[Category: | + | [[Category: Computationally designed protein]] |
| - | [[Category: | + | [[Category: Rrm]] |
| - | [[Category: | + | [[Category: U1a]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:33:26 2008'' | |
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Revision as of 15:33, 3 May 2008
Structure of URNdesign, a complete computational redesign of human U1A protein
Overview
Achieving atomic-level resolution in the computational design of a protein structure remains a challenging problem despite recent progress. Rigorous experimental tests are needed to improve protein design algorithms, yet studies of the structure and dynamics of computationally designed proteins are very few. The NMR structure and backbone dynamics of a redesigned protein of 96 amino acids are compared here with the design target, human U1A protein. We demonstrate that the redesigned protein reproduces the target structure to within the uncertainty of the NMR coordinates, even as 65 out of 96 amino acids were simultaneously changed by purely computational methods. The dynamics of the backbone of the redesigned protein also mirror those of human U1A, suggesting that the protein design algorithm captures the shape of the potential energy landscape in addition to the local energy minimum.
About this Structure
2A3J is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
High-resolution structural validation of the computational redesign of human U1A protein., Dobson N, Dantas G, Baker D, Varani G, Structure. 2006 May;14(5):847-56. PMID:16698546 Page seeded by OCA on Sat May 3 18:33:26 2008
