2a3v

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[[Image:2a3v.gif|left|200px]]
[[Image:2a3v.gif|left|200px]]
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{{Structure
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|PDB= 2a3v |SIZE=350|CAPTION= <scene name='initialview01'>2a3v</scene>, resolution 2.8&Aring;
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The line below this paragraph, containing "STRUCTURE_2a3v", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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|GENE= intI4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243277 Vibrio cholerae O1 biovar eltor str. N16961])
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|DOMAIN=
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{{STRUCTURE_2a3v| PDB=2a3v | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a3v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3v OCA], [http://www.ebi.ac.uk/pdbsum/2a3v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a3v RCSB]</span>
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'''Structural basis for broad DNA-specificity in integron recombination'''
'''Structural basis for broad DNA-specificity in integron recombination'''
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[[Category: MacDonald, D.]]
[[Category: MacDonald, D.]]
[[Category: Mazel, D.]]
[[Category: Mazel, D.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:34:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:47:30 2008''
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Revision as of 15:34, 3 May 2008

Image:2a3v.gif

Template:STRUCTURE 2a3v

Structural basis for broad DNA-specificity in integron recombination


Overview

Lateral DNA transfer--the movement of genetic traits between bacteria--has a profound impact on genomic evolution and speciation. The efficiency with which bacteria incorporate genetic information reflects their capacity to adapt to changing environmental conditions. Integron integrases are proteins that mediate site-specific DNA recombination between a proximal primary site (attI) and a secondary target site (attC) found within mobile gene cassettes encoding resistance or virulence factors. The lack of sequence conservation among attC sites has led to the hypothesis that a sequence-independent structural recognition determinant must exist within attC. Here we report the crystal structure of an integron integrase bound to an attC substrate. The structure shows that DNA target site recognition and high-order synaptic assembly are not dependent on canonical DNA but on the position of two flipped-out bases that interact in cis and in trans with the integrase. These extrahelical bases, one of which is required for recombination in vivo, originate from folding of the bottom strand of attC owing to its imperfect internal dyad symmetry. The mechanism reported here supports a new paradigm for how sequence-degenerate single-stranded genetic material is recognized and exchanged between bacteria.

About this Structure

2A3V is a Single protein structure of sequence from Vibrio cholerae o1 biovar eltor str. n16961. Full crystallographic information is available from OCA.

Reference

Structural basis for broad DNA-specificity in integron recombination., MacDonald D, Demarre G, Bouvier M, Mazel D, Gopaul DN, Nature. 2006 Apr 27;440(7088):1157-62. PMID:16641988 Page seeded by OCA on Sat May 3 18:34:10 2008

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