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2a5h

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[[Image:2a5h.gif|left|200px]]
[[Image:2a5h.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2a5h |SIZE=350|CAPTION= <scene name='initialview01'>2a5h</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2a5h", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=LYS:LYSINE'>LYS</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5&#39;-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysine_2,3-aminomutase Lysine 2,3-aminomutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.4.3.2 5.4.3.2] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= KamA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1550 Clostridium subterminale])
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-->
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|DOMAIN=
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{{STRUCTURE_2a5h| PDB=2a5h | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5h OCA], [http://www.ebi.ac.uk/pdbsum/2a5h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a5h RCSB]</span>
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}}
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'''2.1 Angstrom X-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale SB4, with Michaelis analog (L-alpha-lysine external aldimine form of pyridoxal-5'-phosphate).'''
'''2.1 Angstrom X-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale SB4, with Michaelis analog (L-alpha-lysine external aldimine form of pyridoxal-5'-phosphate).'''
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[[Category: Ruzicka, F J.]]
[[Category: Ruzicka, F J.]]
[[Category: 4fe4]]
[[Category: 4fe4]]
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[[Category: alpha-beta channel]]
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[[Category: Alpha-beta channel]]
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[[Category: external aldimine]]
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[[Category: External aldimine]]
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[[Category: four-iron-four-sulfur cluster]]
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[[Category: Four-iron-four-sulfur cluster]]
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[[Category: fs4]]
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[[Category: Fs4]]
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[[Category: michaelis analog]]
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[[Category: Michaelis analog]]
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[[Category: pyridoxal-5'-phosphate]]
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[[Category: Pyridoxal-5'-phosphate]]
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[[Category: radical sam]]
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[[Category: Radical sam]]
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[[Category: s-adenosylmethionine]]
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[[Category: S-adenosylmethionine]]
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[[Category: sam]]
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[[Category: Sam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:37:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:06 2008''
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Revision as of 15:37, 3 May 2008

Image:2a5h.gif

Template:STRUCTURE 2a5h

2.1 Angstrom X-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale SB4, with Michaelis analog (L-alpha-lysine external aldimine form of pyridoxal-5'-phosphate).


Overview

The x-ray crystal structure of the pyridoxal-5'-phosphate (PLP), S-adenosyl-L-methionine (SAM), and [4Fe-4S]-dependent lysine-2,3-aminomutase (LAM) of Clostridium subterminale has been solved to 2.1-A resolution by single-wavelength anomalous dispersion methods on a L-selenomethionine-substituted complex of LAM with [4Fe-4S]2+, PLP, SAM, and L-alpha-lysine, a very close analog of the active Michaelis complex. The unit cell contains a dimer of hydrogen-bonded, domain-swapped dimers, the subunits of which adopt a fold that contains all three cofactors in a central channel defined by six beta/alpha structural units. Zinc coordination links the domain-swapped dimers. In each subunit, the solvent face of the channel is occluded by an N-terminal helical domain, with the opposite end of the channel packed against the domain-swapped subunit. Hydrogen-bonded ionic contacts hold the external aldimine of PLP and L-alpha-lysine in position for abstraction of the 3-pro-R hydrogen of lysine by C5' of SAM. The structure of the SAM/[4Fe-4S] complex confirms and extends conclusions from spectroscopic studies of LAM and shows selenium in Se-adenosyl-L-selenomethionine poised to ligate the unique iron in the [4Fe-4S] cluster upon electron transfer and radical formation. The chain fold in the central domain is in part analogous to other radical-SAM enzymes.

About this Structure

2A5H is a Single protein structure of sequence from Clostridium subterminale. Full crystallographic information is available from OCA.

Reference

The x-ray crystal structure of lysine-2,3-aminomutase from Clostridium subterminale., Lepore BW, Ruzicka FJ, Frey PA, Ringe D, Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13819-24. Epub 2005 Sep 15. PMID:16166264 Page seeded by OCA on Sat May 3 18:37:55 2008

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