2a6h

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[[Image:2a6h.gif|left|200px]]
[[Image:2a6h.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2a6h |SIZE=350|CAPTION= <scene name='initialview01'>2a6h</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_2a6h", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STD:STREPTOLYDIGIN'>STD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2a6h| PDB=2a6h | SCENE= }}
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|RELATEDENTRY=[[1iw7|1IW7]], [[1smy|1SMY]], [[2a68|2A68]], [[2a69|2A69]], [[2a6e|2A6E]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a6h OCA], [http://www.ebi.ac.uk/pdbsum/2a6h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2a6h RCSB]</span>
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}}
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'''Crystal structure of the T. thermophilus RNA polymerase holoenzyme in complex with antibiotic sterptolydigin'''
'''Crystal structure of the T. thermophilus RNA polymerase holoenzyme in complex with antibiotic sterptolydigin'''
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[[Category: Zenkin, N.]]
[[Category: Zenkin, N.]]
[[Category: Zorov, S.]]
[[Category: Zorov, S.]]
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[[Category: antibiotic]]
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[[Category: Antibiotic]]
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[[Category: riken structural genomics/proteomics initiative]]
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[[Category: Riken structural genomics/proteomics initiative]]
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[[Category: rna polymerase holoenzyme]]
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[[Category: Rna polymerase holoenzyme]]
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[[Category: rsgi]]
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[[Category: Rsgi]]
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[[Category: streptolydigin]]
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[[Category: Streptolydigin]]
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[[Category: structural genomic]]
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[[Category: Structural genomic]]
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[[Category: transcription regulation]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:40:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:48:30 2008''
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Revision as of 15:40, 3 May 2008

Image:2a6h.gif

Template:STRUCTURE 2a6h

Crystal structure of the T. thermophilus RNA polymerase holoenzyme in complex with antibiotic sterptolydigin


Overview

Streptolydigin (Stl) is a potent inhibitor of bacterial RNA polymerases (RNAPs). The 2.4 A resolution structure of the Thermus thermophilus RNAP-Stl complex showed that, in full agreement with the available genetic data, the inhibitor binding site is located 20 A away from the RNAP active site and encompasses the bridge helix and the trigger loop, two elements that are considered to be crucial for RNAP catalytic center function. Structure-based biochemical experiments revealed additional determinants of Stl binding and demonstrated that Stl does not affect NTP substrate binding, DNA translocation, and phosphodiester bond formation. The RNAP-Stl complex structure, its comparison with the closely related substrate bound eukaryotic transcription elongation complexes, and biochemical analysis suggest an inhibitory mechanism in which Stl stabilizes catalytically inactive (preinsertion) substrate bound transcription intermediate, thereby blocking structural isomerization of RNAP to an active configuration. The results provide a basis for a design of new antibiotics utilizing the Stl-like mechanism.

About this Structure

2A6H is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural basis of transcription inhibition by antibiotic streptolydigin., Temiakov D, Zenkin N, Vassylyeva MN, Perederina A, Tahirov TH, Kashkina E, Savkina M, Zorov S, Nikiforov V, Igarashi N, Matsugaki N, Wakatsuki S, Severinov K, Vassylyev DG, Mol Cell. 2005 Sep 2;19(5):655-66. PMID:16167380 Page seeded by OCA on Sat May 3 18:40:09 2008

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