2aay

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[[Image:2aay.gif|left|200px]]
[[Image:2aay.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2aay |SIZE=350|CAPTION= <scene name='initialview01'>2aay</scene>, resolution 1.55&Aring;
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The line below this paragraph, containing "STRUCTURE_2aay", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=GPJ:GLYPHOSATE'>GPJ</scene>, <scene name='pdbligand=SKM:(3R,4S,5R)-3,4,5-TRIHYDROXYCYCLOHEX-1-ENE-1-CARBOXYLIC+ACID'>SKM</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3-phosphoshikimate_1-carboxyvinyltransferase 3-phosphoshikimate 1-carboxyvinyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.19 2.5.1.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= aroA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_2aay| PDB=2aay | SCENE= }}
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|RELATEDENTRY=[[1g6s|1G6S]], [[1g6t|1G6T]], [[1q36|1Q36]], [[1mi4|1MI4]], [[1x8r|1X8R]], [[1x8t|1X8T]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2aay FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2aay OCA], [http://www.ebi.ac.uk/pdbsum/2aay PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2aay RCSB]</span>
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}}
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'''EPSP synthase liganded with shikimate and glyphosate'''
'''EPSP synthase liganded with shikimate and glyphosate'''
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[[Category: Healy, M L.]]
[[Category: Healy, M L.]]
[[Category: Priestman, M A.]]
[[Category: Priestman, M A.]]
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[[Category: inside-out alpha/beta barrel]]
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[[Category: Inside-out alpha/beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:49:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:50:17 2008''
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Revision as of 15:49, 3 May 2008

Image:2aay.gif

Template:STRUCTURE 2aay

EPSP synthase liganded with shikimate and glyphosate


Overview

The shikimate pathway enzyme 5-enolpyruvyl shikimate-3-phosphate synthase (EPSP synthase) has received attention in the past because it is the target of the broad-spectrum herbicide glyphosate. The natural substrate of EPSP synthase is shikimate-3-phosphate. However, this enzyme can also utilize shikimate as substrate. Remarkably, this reaction is insensitive to inhibition by glyphosate. Crystallographic analysis of EPSP synthase from Escherichia coli, in complex with shikimate/glyphosate at 1.5 Angstroms resolution, revealed that binding of shikimate induces changes around the backbone of the active site, which in turn impact the efficient binding of glyphosate. The implications from these findings with respect to the design of novel glyphosate-insensitive EPSP synthase enzymes are discussed.

About this Structure

2AAY is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Molecular basis for the glyphosate-insensitivity of the reaction of 5-enolpyruvylshikimate 3-phosphate synthase with shikimate., Priestman MA, Healy ML, Funke T, Becker A, Schonbrunn E, FEBS Lett. 2005 Oct 24;579(25):5773-80. PMID:16225867 Page seeded by OCA on Sat May 3 18:49:51 2008

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OCA

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