2afn
From Proteopedia
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[[Image:2afn.gif|left|200px]] | [[Image:2afn.gif|left|200px]] | ||
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'''STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC''' | '''STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2AFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. This structure supersedes the now removed PDB entry | + | 2AFN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1afn 1afn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AFN OCA]. |
==Reference== | ==Reference== | ||
Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc., Murphy ME, Turley S, Kukimoto M, Nishiyama M, Horinouchi S, Sasaki H, Tanokura M, Adman ET, Biochemistry. 1995 Sep 26;34(38):12107-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7547950 7547950] | Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc., Murphy ME, Turley S, Kukimoto M, Nishiyama M, Horinouchi S, Sasaki H, Tanokura M, Adman ET, Biochemistry. 1995 Sep 26;34(38):12107-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7547950 7547950] | ||
[[Category: Alcaligenes faecalis]] | [[Category: Alcaligenes faecalis]] | ||
| - | [[Category: Nitrite reductase (NO-forming)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Adman, E T.]] | [[Category: Adman, E T.]] | ||
[[Category: Murphy, M E.P.]] | [[Category: Murphy, M E.P.]] | ||
[[Category: Turley, S.]] | [[Category: Turley, S.]] | ||
| - | [[Category: | + | [[Category: Copper]] |
| - | [[Category: | + | [[Category: Fad]] |
| - | [[Category: | + | [[Category: Flavoprotein]] |
| - | [[Category: | + | [[Category: Nitrite assimilation]] |
| - | + | [[Category: Oxidoreductase]] | |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 18:59:40 2008'' |
| - | + | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 15:59, 3 May 2008
STRUCTURE OF ALCALIGENES FAECALIS NITRITE REDUCTASE AND A COPPER SITE MUTANT, M150E, THAT CONTAINS ZINC
Overview
The structures at 2.0 and 2.25 A resolution of native and recombinant nitrite reductase from Alcaligenes faecalis show that they are identical to each other and very similar to nitrite reductase from Achromobacter cycloclastes. The crystallographic structure of a mutant, M150E, which unlike the wild-type protein cannot be reduced by pseudoazurin, shows that the glutamate replacement for methionine binds to a metal at the type I Cu site via only one oxygen. Anomalous scattering data collected at wavelengths of 1.040 and 1.377 A reveal that the metal at the type I site is a Zn. No significant differences from the native structure other than local perturbations at the type I site are seen. A local pseudo 2-fold axis relates the two domains of different monomers which form the active site. The two residues, Asp98 and His255, believed to be involved in catalysis are related by this 2-fold. An unusual (+)-(+) charge interaction between Lys269, Glu279, and His100 helps to orient the active site Cu ligand, His100. A number of negatively charged surface residues create an electrostatic field whose shape suggests that it may serve to direct incoming negatively charged nitrite as well as to dock the electron donor partner, pseudoazurin.
About this Structure
2AFN is a Single protein structure of sequence from Alcaligenes faecalis. This structure supersedes the now removed PDB entry 1afn. Full crystallographic information is available from OCA.
Reference
Structure of Alcaligenes faecalis nitrite reductase and a copper site mutant, M150E, that contains zinc., Murphy ME, Turley S, Kukimoto M, Nishiyama M, Horinouchi S, Sasaki H, Tanokura M, Adman ET, Biochemistry. 1995 Sep 26;34(38):12107-17. PMID:7547950 Page seeded by OCA on Sat May 3 18:59:40 2008
