2agh
From Proteopedia
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'''Structural basis for cooperative transcription factor binding to the CBP coactivator''' | '''Structural basis for cooperative transcription factor binding to the CBP coactivator''' | ||
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[[Category: Guzman, R N.De.]] | [[Category: Guzman, R N.De.]] | ||
[[Category: Wright, P E.]] | [[Category: Wright, P E.]] | ||
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| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:01:13 2008'' | |
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Revision as of 16:01, 3 May 2008
Structural basis for cooperative transcription factor binding to the CBP coactivator
Overview
Regulation of transcription requires interactions between transcriptional activators and transcriptional co-activator CREB binding protein (CBP). The KIX domain of CBP can bind simultaneously to two different proteins, providing an additional mechanism for transcriptional regulation. Here we describe the solution structure of the ternary complex formed by cooperative binding of activation domains from the c-Myb and mixed lineage leukemia (MLL) transcription factors to the KIX domain. The MLL and c-Myb domains form helices that bind to two distinct hydrophobic grooves on opposite faces of KIX. Compared to the binary KIX:c-Myb complex, significant changes are observed in the structure of KIX at the MLL binding interface in the ternary complex. Two regions of KIX that are disordered in the binary complex become structured in the ternary complex: a flexible loop forms intimate contacts with bound MLL, and the C-terminal helix is extended and stabilized by MLL binding. This structural change results in the formation of additional electrostatic/polar interactions between KIX and the bound c-Myb, providing a structural basis for the cooperativity observed for the ternary complex.
About this Structure
2AGH is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis for cooperative transcription factor binding to the CBP coactivator., De Guzman RN, Goto NK, Dyson HJ, Wright PE, J Mol Biol. 2006 Feb 3;355(5):1005-13. Epub 2005 Oct 5. PMID:16253272 Page seeded by OCA on Sat May 3 19:01:13 2008
